Wang Lin-Lin, Tang Ping-Hua, Shi Chao-Guo, Wan Ying-Huan, Tang Wei, Hou Xia-Xiao, Pan Na-Li, Shi Yu-Bao, Tao Qin-Lian
Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.
Department of Pulmonary Medicine, Qingdao Municipal Hospital, Qingdao University School of Medicine, Qingdao, Shandong 266011.
Biomed Rep. 2014 Jan;2(1):75-78. doi: 10.3892/br.2013.196. Epub 2013 Nov 6.
The ectoenzyme CD39 hydrolyzes extracellular adenosine 5'-triphosphate (ATP), which possesses pro-inflammatory properties. However, the role of CD39 in allergic asthma has not been fully elucidated. A total of 18 patients with persistent asthma who were allergic to house dust mites and 19 healthy volunteers were enrolled in this study. The expression of CD39, GATA3, RAR-related orphan receptor γ (ROR-γt) and forkhead box P3 (FoxP3) mRNA in peripheral blood mononuclear cells (PBMCs) was determined by SYBR-Green I quantitative polymerase chain reaction (qPCR). The cytokines interleukin (IL)-4, IL-17A, transforming growth factor β (TGF-β) and DP.sIgE were detected by enzyme-linked immunosorbent assay. Our data demonstrated that the expression of CD39 mRNA in PBMCs from asthmatic patients was significantly lower compared to that in normal controls [(1.49±0.59)×10 vs. (2.17±0.77)×10, respectively; P<0.01]. CD39 mRNA was negatively correlated with serum IL-4, IL-17A and GATA3 expression (r=-0.468, P<0.05; r=-0.550, P<0.05; and r=-0.424, P<0.01, respectively) and positively correlated with FoxP3 and TGF-β expression (r=0.373, P<0.05; and r=0.425, P<0.05, respectively). There was no obvious correlation between CD39 and ROR-γt expression (r=-0.259, P=0.122). These data suggested that CD39 mRNA expression was downregulated in allergic asthma, which was positively correlated with serum IL-4, IL-17A and GATA3 expression and negatively correlated with serum TGF-β and FoxP3 expression, whereas there was no correlation with ROR-γt. Therefore, it was hypothesized that CD39 may participate in the occurrence and progression of allergic asthma.
胞外酶CD39可水解具有促炎特性的细胞外三磷酸腺苷（ATP）。然而，CD39在过敏性哮喘中的作用尚未完全阐明。本研究共纳入了18名对屋尘螨过敏的持续性哮喘患者和19名健康志愿者。采用SYBR - Green I定量聚合酶链反应（qPCR）测定外周血单个核细胞（PBMC）中CD39、GATA3、视黄酸相关孤儿受体γ（ROR - γt）和叉头框蛋白P3（FoxP3）mRNA的表达。通过酶联免疫吸附测定法检测细胞因子白细胞介素（IL）-4、IL -17A、转化生长因子β（TGF - β）和DP.sIgE。我们的数据表明，哮喘患者PBMC中CD39 mRNA的表达明显低于正常对照组[分别为(1.49±0.59)×10与(2.17±0.77)×10；P<0.01]。CD39 mRNA与血清IL -4、IL -17A和GATA3表达呈负相关（r分别为-0.468，P<0.05；-0.550，P<0.05；-0.424，P<0.01），与FoxP3和TGF - β表达呈正相关（r分别为0.373，P<0.05；0.425，P<0.05）。CD39与ROR - γt表达之间无明显相关性（r = -0.259，P = 0.122）。这些数据表明，过敏性哮喘中CD39 mRNA表达下调，其与血清IL -4、IL -17A和GATA3表达呈正相关，与血清TGF - β和FoxP3表达呈负相关，而与ROR - γt无相关性。因此，推测CD39可能参与过敏性哮喘的发生和发展。
