Qiao En-Qi, Ji Minghua, Wu Jianzhong, Li Jian, Xu Xinyu, Ma Rong, Zhang Xiaohua, He Yuejun, Zha Quanbin, Song Xue, Zhu Liwei, Tang Ji-Hai
Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
Radiotherapy, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
Mol Clin Oncol. 2013 Jul;1(4):703-710. doi: 10.3892/mco.2013.111. Epub 2013 May 8.
Breast cancer is one of the most common malignancies in women. This study was conducted to analyze the association between the expressions of eight immunohistochemical (IHC) indices and clinicopathological characteristics in breast cancers (BCs) and investigate the clinical significance. IHC Envision ldpe-g-nvp was used to detect the expression of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER2), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), p53, type II topoisomerase (TOPO II) and Ki-67 in postoperative paraffin blocks of 286 cases of invasive BC and statistically analyzed their correlations with clinicopathological characteristics. The positive rates of ER, PR, HER2, VEGF, p53, EGFR, TOPO II and Ki-67 expression were 62.24, 41.96, 57.34, 53.85, 81.82, 46.85, 54.55 and 69.93%, respectively. ER expression was negatively correlated with age, tumor size and histological grade (P<0.05) and PR expression was negatively correlated with age and histological grade (P<0.05). Among the ER, PR and c-erbB-2 statuses, a significant correlation was observed between ER expression and PR status (P=0.0000), whereas the expression of ER and PR exhibited a negative correlation with HER2 status (P<0.05). We also demonstrated a significant correlation between EGFR expression and lymph node metastasis (P=0.0240), p53 expression and tumor size (P=0.0300), p53 and Ki-67 expression and histological grade (P<0.05) and the expressions of VEGF, EGFR, p53, TOPO II, Ki-67 and HER2 status (P<0.05). In addition, the Luminal B and HER2/neu subtypes exhibited a close correlation with age (P<0.01), while the HER2/neu and triple-negative subtypes were positively correlated with poor histological grade (P<0.05). In conclusion, there is a definite correlation between IHC indices and clinicopathological characteristics in BCs. Combined detection of these indices may be significant in the evaluation of biological behavior and prognosis of BC and thus in the diagnosis and comprehensive treatment of this disease.
乳腺癌是女性最常见的恶性肿瘤之一。本研究旨在分析8种免疫组化（IHC）指标的表达与乳腺癌（BC）临床病理特征之间的关联，并探讨其临床意义。采用IHC Envision ldpe-g-nvp检测286例浸润性BC术后石蜡块中雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体2（HER2）、血管内皮生长因子（VEGF）、表皮生长因子受体（EGFR）、p53、II型拓扑异构酶（TOPO II）和Ki-67的表达，并对其与临床病理特征的相关性进行统计学分析。ER、PR、HER2、VEGF、p53、EGFR、TOPO II和Ki-67表达的阳性率分别为62.24%、41.96%、57.34%、53.85%、81.82%、46.85%、54.55%和69.93%。ER表达与年龄、肿瘤大小和组织学分级呈负相关（P<0.05），PR表达与年龄和组织学分级呈负相关（P<0.
