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基质辅助激光解析电离飞行时间质谱法检测脂肪肝模型中三酰甘油结构和分布图谱的变化。

Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF.

机构信息

Department of Traumatology and Critical Care Medicine, National Defense Medical College, Saitama 359-8513, Japan.

JEOL Ltd., Akishima, Tokyo 196-8553, Japan.

出版信息

FEBS Open Bio. 2014 Feb 19;4:179-84. doi: 10.1016/j.fob.2014.02.005. eCollection 2014.

DOI:10.1016/j.fob.2014.02.005
PMID:24649399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3953719/
Abstract

Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.

摘要

基质辅助激光解吸/电离螺旋轨道飞行时间质谱（MALDI-SpiralTOF）可分析脂质谱并表征脂质结构。成像质谱（IMS）还提供了选定 m/z 值的分布图谱。在这里，我们使用这些技术研究了三酰基甘油（TG）的结构和分布，以评估小鼠脂肪肝。IMS 在 m/z 881.7（52:2）处指示了最强烈的质谱离子的分布和强度。MS/MS 分析表明肝 TG 和饮食 TG 之间存在结构变化。这些发现表明 MALDI-SpiralTOF 是临床筛选和估计脂肪肝的有力工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/398e148c7751/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/cdb5a312c051/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/c58921c6eb4b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/ec1c6d002e00/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/398e148c7751/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/cdb5a312c051/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/c58921c6eb4b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/ec1c6d002e00/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/504e/3953719/398e148c7751/gr4.jpg

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