Doerr R, Castillo M, Evans P, Paolini N, Goldrosen M, Cohen S A
Department of Surgery, State University of New York, Buffalo.
Arch Surg. 1989 Feb;124(2):170-4. doi: 10.1001/archsurg.1989.01410020040006.
Despite adequate locoregional control, colorectal metastasis to the liver remains a significant cause of death. Resection of hepatic metastasis improves five-year survival 18% to 34%. A study of the impact of 40% partial hepatectomy on cytokine production in the liver was undertaken. Nonparenchymal liver cells (NPCs) were prepared by collagenase perfusion and metrizamide gradient from partially hepatectomized and laparotomized control C57BL/6Ros mice. Nonparenchymal cell from partially hepatectomized mice compared with laparotomized mice showed a twofold to threefold increase in interferon (IFN) activity. Both interferon alpha/beta and supernatants from cultured NPCs of partially hepatectomized mice suppressed the proliferation of liver-derived MCA-38 colon adenocarcinoma cells in vitro. This tumor has been shown to metastasize to the liver of C57BL/6Ros mice. The production of various cytokines by NPCs induced by partial hepatectomy may provide a possible antimetastatic mechanism.
尽管局部区域控制良好，但结直肠癌肝转移仍是一个重要的死亡原因。肝转移灶切除可使五年生存率提高18%至34%。本研究探讨了40%肝部分切除术对肝脏细胞因子产生的影响。通过胶原酶灌注和Metrizamide梯度法，从部分肝切除和剖腹手术对照的C57BL/6Ros小鼠中制备非实质肝细胞（NPC）。与剖腹手术小鼠相比，部分肝切除小鼠的非实质细胞中干扰素（IFN）活性增加了两倍至三倍。部分肝切除小鼠的干扰素α/β以及培养的NPC上清液均可在体外抑制肝源性MCA-38结肠腺癌细胞的增殖。该肿瘤已被证明可转移至C57BL/6Ros小鼠的肝脏。部分肝切除诱导的NPC产生的各种细胞因子可能提供了一种可能的抗转移机制。
