Mehta Angela, López-Maury Luis, Florencio Francisco J
Embrapa Recursos Genéticos e Biotecnologia, Av. W5 Norte (final), 70770-917 Brasília, DF, Brazil.
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, E-41092 Seville, Spain.
J Proteomics. 2014 May 6;102:98-112. doi: 10.1016/j.jprot.2014.03.002. Epub 2014 Mar 17.
Cyanobacteria represent the largest and most diverse group of prokaryotes capable of performing oxygenic photosynthesis and are frequently found in environments contaminated with heavy metals. Several studies have been performed in these organisms in order to better understand the effects of metals such as Zn, Cd, Cu, Ni and Co. In Synechocystis sp. PCC 6803, genes involved in Ni, Co, Cu and Zn resistance have been reported. However, proteomic studies for the identification of proteins modulated by heavy metals have not been carried out. In the present work, we have analyzed the proteomic pattern alterations of the cyanobacterium Synechocystis sp. PCC 6803 in response to Ni, Co and Cd in order to identify the metabolic processes affected by these metals. We show that some proteins are commonly regulated in response to the different metal ions, including ribulose1,5-bisphosphate carboxylase and the periplasmic iron-binding protein FutA2, while others, such as chaperones, were specifically induced by each metal. We also show that the main processes affected by the metals are carbon metabolism and photosynthesis, since heavy metals affect proteins required for the correct functioning of these activities.
This is the first report on the proteomic profile of Synechocystis sp. PCC 6803 wild type and mutant strains for the identification of proteins affected by the heavy metals Ni, Co and Cd. We have identified proteins commonly responsive to all three metals and also chaperones specifically modulated by each metal. Our data also supports previous studies that suggest the existence of additional sensor systems for Co.
蓝细菌是能够进行产氧光合作用的最大且最多样化的原核生物群体，并且经常在被重金属污染的环境中被发现。为了更好地理解锌、镉、铜、镍和钴等金属的影响，已经对这些生物体进行了多项研究。在集胞藻PCC 6803中，已经报道了参与镍、钴、铜和锌抗性的基因。然而，尚未开展用于鉴定受重金属调节的蛋白质的蛋白质组学研究。在本研究中，我们分析了集胞藻PCC 6803对镍、钴和镉的蛋白质组模式变化，以确定受这些金属影响的代谢过程。我们发现，一些蛋白质在对不同金属离子的响应中受到共同调节，包括1,5-二磷酸核酮糖羧化酶和周质铁结合蛋白FutA2，而其他蛋白质，如伴侣蛋白，则由每种金属特异性诱导。我们还表明，受金属影响的主要过程是碳代谢和光合作用，因为重金属会影响这些活动正常运作所需的蛋白质。
这是关于集胞藻PCC 6803野生型和突变株蛋白质组图谱的首篇报道，用于鉴定受重金属镍、钴和镉影响的蛋白质。我们已经鉴定出对所有三种金属都有响应的蛋白质以及由每种金属特异性调节的伴侣蛋白。我们的数据也支持了先前的研究，这些研究表明存在针对钴的额外传感系统。
