Newton B W, Burkhart A B, Romagnano M A, Hamill R W
Neurology Unit, Monroe Community Hospital, Rochester, NY 14603.
Brain Res Dev Brain Res. 1988 Dec 1;44(2):161-80. doi: 10.1016/0165-3806(88)90215-5.
The ontogeny of substance P (SP)-containing fibers and puncta is described in laminae VII and X of the rat thoracolumbar spinal cord from the day of birth until postnatal day (P) 60. As SP fibers and puncta innervate and demarcate the distribution of preganglionic sympathetic nuclei, strong temporal and weak rostrocaudal ontogenetic gradients exist. Additionally, a heterogeneous segmental SP ontogenetic pattern is observed in sympathetic nuclei. On the day of birth, SP fibers are present in an unorganized fashion in sympathetic nuclei with the exception of the nucleus intercalatus which is clearly outlined. From P0 to P4 SP fibers and puncta are established along a 'ladder-like' pattern and from P6 to P15 SP fibers and puncta steadily accumulate in sympathetic nuclei at all spinal levels. By P15 the nuclei intermediolateralis, pars principalis and funicularis, and the nucleus intercalatus are clearly outlined by SP immunoreactivity while the central autonomic region (lamina X) contains heterogeneous bands of SP immunoreactivity. From P20 to P30, SP fibers and puncta accumulate in each autonomic nucleus and longitudinal SP connections form between each adjacent nucleus intermediolateralis pars principalis. Along the spinal midline the nucleus intercalatus pars paraependymalis and the dorsal commissural nucleus emerge from the central autonomic region as separate SP-innervated nuclei. On P40 a period of reorganization takes place so that SP fibers within sympathetic nuclei become more diffuse and the nucleus intercalatus becomes more complex in appearance. The adult SP pattern is formed by P60 when the dorsal commissural nucleus dense core becomes the last autonomic region to be SP innervated. In addition, a transient population of immunohistochemically demonstrable cervical-thoracic laminae VII and X SP cell bodies are observed from P2 to P15.
描述了从出生日至出生后第60天大鼠胸腰段脊髓VII层和X层中含P物质（SP）纤维和终扣的个体发生情况。由于SP纤维和终扣支配并划分了节前交感神经核的分布，因此存在强烈的时间性和较弱的尾头个体发生梯度。此外，在交感神经核中观察到异质性节段性SP个体发生模式。出生当天，SP纤维以无序方式存在于交感神经核中，但闰核清晰可见。从出生后第0天到第4天，SP纤维和终扣沿“梯状”模式形成，从出生后第6天到第15天，SP纤维和终扣在所有脊髓节段的交感神经核中稳定积累。到出生后第15天，中间外侧核、主部和索部以及闰核被SP免疫反应清晰勾勒出来，而中央自主区（X层）含有异质性SP免疫反应带。从出生后第20天到第30天，SP纤维和终扣在每个自主神经核中积累，相邻中间外侧核主部之间形成纵向SP连接。沿着脊髓中线，室管膜旁闰核和背连合核从中央自主区分离出来，成为单独的SP支配核。在出生后第40天发生一段重组期，使得交感神经核内的SP纤维变得更加弥散，闰核外观变得更加复杂。成年SP模式在出生后第60天形成，此时背连合核致密核心成为最后一个被SP支配的自主区。此外，在出生后第2天到第15天观察到一群免疫组化可显示的颈胸段VII层和X层SP细胞体。
