Walton P E, Etherton T D
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
J Endocrinol. 1989 Jan;120(1):153-60. doi: 10.1677/joe.0.1200153.
A specific radioimmunoassay (RIA) was established for an acid-stable insulin-like growth factor-binding protein (IGF-BP) isolated from porcine serum. The RIA recognizes a GH-dependent 150 kDa protein in porcine serum; therefore we postulate that the acid-stable IGF-BP is a component of the high molecular weight IGF-BP in porcine serum. The IGF-BP concentration was assayed in porcine serum (normal, 2.44 mg/l; hypophysectomized, 0.83 mg/l; serum from a GH-treated pig, 4.72 mg/l), porcine colostrum (2.55 mg/l), milk (0.91 mg/l), and amniotic (1.82-3.14 mg/l), allantoic (2.94-3.58 mg/l) and follicular (2.28 mg/l) fluids. Serum concentrations of IGF-BP were significantly increased (63%) in pigs chronically injected with porcine GH (pGH) (70 micrograms/kg body weight per day for 17 days). Concentrations of IGF-BP did not change in porcine serum following acute challenges with pGH (10-1000 micrograms/kg body weight) or IGF-I (4 or 8 mg per pig). This is the first report of a specific RIA for the porcine GH-dependent IGF-BP. Our results indicate that this IGF-BP is found in a wide variety of biological fluids and that its concentration appears to be regulated by pGH but not by IGF-I.
建立了一种针对从猪血清中分离出的酸稳定胰岛素样生长因子结合蛋白（IGF - BP）的特异性放射免疫分析（RIA）方法。该RIA可识别猪血清中一种生长激素依赖性150 kDa蛋白；因此我们推测，酸稳定的IGF - BP是猪血清中高分子量IGF - BP的一个组成部分。对猪血清（正常，2.44 mg/l；垂体切除后，0.83 mg/l；经生长激素处理的猪的血清，4.72 mg/l）、猪初乳（2.55 mg/l）、牛奶（0.91 mg/l）以及羊水（1.82 - 3.14 mg/l）、尿囊液（2.94 - 3.58 mg/l）和卵泡液（2.28 mg/l）中的IGF - BP浓度进行了测定。长期注射猪生长激素（pGH）（每天70微克/千克体重，共17天）的猪血清中IGF - BP浓度显著升高（63%）。用pGH（10 - 1000微克/千克体重）或IGF - I（每头猪4或8毫克）急性刺激后，猪血清中IGF - BP浓度未发生变化。这是关于猪生长激素依赖性IGF - BP特异性RIA的首次报道。我们的结果表明，这种IGF - BP存在于多种生物体液中，其浓度似乎受pGH调节，但不受IGF - I调节。
