Carlomagno Teresa
European Molecular Biology Laboratory, Structural and Computational Biology Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
J Magn Reson. 2014 Apr;241:126-36. doi: 10.1016/j.jmr.2013.10.007.
Nucleic acids are gaining enormous importance as key molecules in almost all biological processes. Most nucleic acids do not act in isolation but are generally associated with proteins to form high-molecular-weight nucleoprotein complexes. In this perspective article I focus on the structural studies of supra-molecular ribonucleoprotein (RNP) assemblies in solution by a combination of state-of-the-art TROSY-based NMR experiments and other structural biology techniques. I discuss ways how to combine sparse NMR data with low-resolution structural information from small-angle scattering, fluorescence and electron paramagnetic resonance spectroscopy to obtain the structure of large RNP particles by an integrated structural biology approach. In the last section I give a perspective for the study of RNP complexes by solid-state NMR.
核酸作为几乎所有生物过程中的关键分子正变得极其重要。大多数核酸并非单独起作用，而是通常与蛋白质结合形成高分子量的核蛋白复合物。在这篇观点文章中，我重点介绍通过结合基于TROSY的先进核磁共振实验和其他结构生物学技术，对溶液中的超分子核糖核蛋白（RNP）组装体进行结构研究。我讨论了如何将稀疏的核磁共振数据与来自小角散射、荧光和电子顺磁共振光谱的低分辨率结构信息相结合，通过综合结构生物学方法获得大型RNP颗粒的结构。在最后一部分，我对通过固态核磁共振研究RNP复合物给出了展望。
