Johannes Kepler University Linz, Institute for Chemical Technology of Organic Materials, Altenberger Str. 69, 4040 Linz, Austria.
Johannes Kepler University Linz, Institute for Chemical Technology of Organic Materials, Altenberger Str. 69, 4040 Linz, Austria.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:94-100. doi: 10.1016/j.msec.2014.01.050. Epub 2014 Feb 6.
The increasing number of antibiotic-resistant bacterial strains has developed into a major health problem. In particular, biofilms are the main reason for hospital-acquired infections and diseases. Once formed, biofilms are difficult to remove as they have specific defense mechanisms against antimicrobial agents. Antimicrobial surfaces must therefore kill or repel bacteria before they can settle to form a biofilm. In this study, we describe that poly(acrylic acid) (PAA) containing diblock copolymers can kill bacteria and prevent from biofilm formation. The PAA diblock copolymers with poly(styrene) and poly(methyl methacrylate) were synthesized via anionic polymerization of tert-butyl acrylate with styrene or methyl methacrylate and subsequent acid-catalyzed hydrolysis of the tert-butyl ester. The copolymers were characterized via nuclear magnetic resonance spectroscopy (NMR), size-exclusion chromatography (SEC), Fourier transform infrared spectroscopy (FTIR), elemental analysis, and acid-base titrations. Copolymer films with a variety of acrylic acid contents were produced by solvent casting, characterized by atomic force microscopy (AFM) and tested for their antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The antimicrobial activity of the acidic diblock copolymers increased with increasing acrylic acid content, independent of the copolymer-partner, the chain length and the nanostructure.
越来越多的抗生素耐药菌株已发展成为一个主要的健康问题。特别是，生物膜是医院获得性感染和疾病的主要原因。生物膜一旦形成，由于其具有针对抗菌剂的特定防御机制，因此很难去除。因此，抗菌表面必须在细菌定居形成生物膜之前杀死或排斥细菌。在这项研究中，我们描述了含有嵌段共聚物的聚丙烯酸（PAA）可以杀死细菌并防止生物膜形成。通过苯乙烯或甲基丙烯酸甲酯与叔丁基丙烯酸盐的阴离子聚合以及随后叔丁酯的酸催化水解，合成了具有聚苯乙烯和聚甲基丙烯酸甲酯的 PAA 嵌段共聚物。通过核磁共振波谱（NMR）、凝胶渗透色谱（SEC）、傅里叶变换红外光谱（FTIR）、元素分析和酸碱滴定对共聚物进行了表征。通过溶剂浇铸制备了具有各种丙烯酸含量的共聚物薄膜，通过原子力显微镜（AFM）对其进行了表征，并测试了它们对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌活性。酸性嵌段共聚物的抗菌活性随丙烯酸含量的增加而增加，与共聚物伙伴、链长和纳米结构无关。
