Smith J P, Kosco M H, Tew J G, Szakal A K
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Anat Rec. 1988 Dec;222(4):380-90. doi: 10.1002/ar.1092220410.
This study was prompted by the observation that cells in mouse lymph nodes (LN) with cytological characteristics of tingible body macrophages (TBM) appeared to be Thy-1 positive. The objective of this study was to determine if these large cells were TBM and to conclusively demonstrate their reactivity for Thy-1. The cells were studied using monoclonal antibodies (MoAb) against Thy-1 and macrophage markers including F4/80 and Ia antigens at both light microscopic (LM) and electron microscopic (EM) levels. Immunocytochemical reactivity by TBM for Thy-1 antigen specific MoAb was demonstrated by LM in both in situ and in vitro LN preparations. Furthermore, ultrastructural examination of these germinal center cells in situ demonstrated that the Thy-1 reactivity visualized at the LM level was associated with ribosomes and endoplasmic reticulum as well as their plasma membrane. Similarly, these cells expressed intracytoplasmic and membrane reactivity for Ia antigens and also for the macrophage specific antigen F4/80. This indicates that the reactivity is due to active synthesis of the Thy-1 antigen and not attributable to reactivity of any phagocytosed Thy-1 positive cells. As defined by their germinal center location and morphological characteristics, these Thy-1 reactive macrophages were identified as TBM. Germinal center TBM thus represent a unique, vigorously phagocytic subset of mature macrophages which express both macrophage and thymocyte markers.
本研究源于一项观察结果，即小鼠淋巴结（LN）中具有可染体巨噬细胞（TBM）细胞学特征的细胞似乎是Thy-1阳性的。本研究的目的是确定这些大细胞是否为TBM，并确凿地证明它们对Thy-1的反应性。使用针对Thy-1以及包括F4/80和Ia抗原在内的巨噬细胞标志物的单克隆抗体（MoAb），在光学显微镜（LM）和电子显微镜（EM）水平对这些细胞进行研究。在原位和体外LN制剂中，通过LM均证实了TBM对Thy-1抗原特异性MoAb的免疫细胞化学反应性。此外，对这些生发中心细胞的原位超微结构检查表明，在LM水平观察到的Thy-1反应性与核糖体、内质网及其质膜相关。同样，这些细胞对Ia抗原以及巨噬细胞特异性抗原F4/80表现出胞浆内和膜反应性。这表明该反应性是由于Thy-1抗原的活性合成，而不是由于任何吞噬的Thy-1阳性细胞的反应性。根据其生发中心位置和形态特征定义，这些Thy-1反应性巨噬细胞被鉴定为TBM。因此，生发中心TBM代表了成熟巨噬细胞中一个独特的、具有强烈吞噬作用的亚群，其同时表达巨噬细胞和胸腺细胞标志物。
