Smith J P, Lister A M, Tew J G, Szakal A K
Department of Anatomy, Medical College of Virginia/Virginia Commonwealth University, Richmond 23298.
Anat Rec. 1991 Apr;229(4):511-20. doi: 10.1002/ar.1092290412.
Although tingible body macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of tingible bodies or histochemically by using the monoclonal antibody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.
尽管可染体巨噬细胞（TBM）在生发中心已被认识超过100年，但其在生发中心反应中的作用尚不清楚。在本研究中，对年轻小鼠TBM反应的动力学进行了定量评估，并将其与生发中心发育的动力学相关联。为作比较，分析了老年小鼠（其生发中心发育存在与年龄相关的衰退；Szakal等人，1990年）的TBM反应。用人类血清白蛋白对年轻和老年免疫小鼠进行攻击，在0、1、3、5、7、10和14天后评估腘窝和腋窝淋巴结。使用辣根过氧化物酶偶联花生凝集素，通过组织化学方法在交替的连续切片中定位生发中心。通过可染体的存在在相邻切片上确定每个生发中心的TBM数量，或使用单克隆抗体Mac-2通过组织化学方法确定。对年轻小鼠淋巴结的分析表明，TBM数量随着先前存在的生发中心的解离而减少。攻击后5天TBM重新出现，且TBM动力学与新生发中心大小的增加平行。事实上，从第5天到第10天，维持着每350 - 450个B细胞对应一个TBM的恒定比例。在老年淋巴结中，在生发中心发育的整个过程中通常都没有TBM。生发中心发育之前缺乏TBM以及老年小鼠中不存在TBM，这与TBM是生发中心反应诱导所必需的概念不一致。然而，这些数据与TBM在调节生发中心反应强度方面的作用是一致的。
