Hong C Y, Huang J J, Wu P, Lo S J, Wei Y H
Department of Medicine, Taiwan Veterans General Hospital, Taipei, ROC.
Andrologia. 1988 Nov-Dec;20(6):516-20.
We developed a supravital stain of human sperm with fluorescent dyes. Either Hoechst 33,258 or fluorescein isothiocyanate could be used, the former stained sperm head while the later stained the whole sperm. Sperm vitality assessed with any of these two fluorescent dyes correlated well with that determined by eosin-nigrosin counterstain. When sperm vitality was compared with sperm motility measured with a transmembrane migration method, we found that many vital sperm were immotile because sperm vitality was higher than sperm motility in tested samples.
我们研发了一种用荧光染料对人类精子进行的超活染色法。可以使用Hoechst 33258或异硫氰酸荧光素，前者可对精子头部进行染色，而后者可对整个精子进行染色。用这两种荧光染料中的任何一种评估的精子活力与用伊红 - 黑色素复染法测定的结果相关性良好。当将精子活力与用跨膜迁移法测量的精子运动性进行比较时，我们发现许多有活力的精子是不运动的，因为在测试样本中精子活力高于精子运动性。
