Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica.

In order to effectively preserve green spores, which have relatively higher water content and lose viability more quickly than non-green spores, we studied the effect of desiccation level and storage temperature on Osmunda japonica spores. The water content of fresh spores was 11.20%. After 12h desiccation by silica gel, the water content decreased to 6% but spore viability did not change significantly. As the desiccation continued, the decrease in water content slowed, but spore viability dropped. For almost all storage periods, the effects of storage temperature, desiccation level, and temperature×desiccation level were significantly different. After seven days of storage, spores at any desiccation level stored at 4°C obtained high germination rates. After more than seven days storage, liquid nitrogen (LN) storage obtained the best results. Storage at -18°C led to the lowest germination rates. Spores stored at room temperature and -18°C all died within three months. For storage at 4°C and in LN, spores desiccated 12 and 36 h obtained better results. Spores without desiccation had the highest germination rates after being stored at room temperature, but suffered the greatest loss after storage at -18°C. These results suggest that LN storage is the best method of long-term storage of O. japonica spores. The critical water content of O. japonica spores is about 6% and reduction of the water content to this level improves outcome after LN storage greatly. The reason for various responses of O. japonica spores to desiccation and storage temperatures are discussed.