Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China; School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, China.
Neurosci Lett. 2014 May 1;567:6-10. doi: 10.1016/j.neulet.2014.03.003. Epub 2014 Mar 19.
Post-stroke epilepsy (PSE) has a negative effect on stroke prognosis and quality of life. The CD40/CD40L system is reported to be involved in the progression of multiple disease states. We investigated the association between functional polymorphism of CD40 and PSE susceptibility, and we also explored the role of the CD40/CD40L system in PSE. A case-control study was performed in 410 ischemic stroke (IS) patients and in 389 PSE patients. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The CD40 mRNA and protein levels were determined by real-time PCR and western blotting, respectively. The plasma sCD40L level was detected using an ELISA kit. The frequency of the T allele in PSE patients was significantly higher than in IS patients (P<0.05). The plasma sCD40L level was significantly higher in the PSE patients than in the healthy controls and IS patients (P<0.01, P<0.05, respectively). The peripheral blood mononuclear cells (PBMCs) from PSE patients showed significantly higher CD40 mRNA and protein expression than the healthy controls and IS patients (P<0.01, P<0.05, respectively). In the PSE patients, the T-allele carriers showed increased plasma sCD40L levels and increased CD40 mRNA expression. Our study suggested that the T allele of the CD40 -1C/T polymorphism may be associated with PSE susceptibility. The CD40/CD40L system is involved in the process of PSE.
卒中后癫痫（PSE）对卒中预后和生活质量有负面影响。CD40/CD40L 系统被报道参与多种疾病状态的进展。我们研究了 CD40 功能多态性与 PSE 易感性之间的关联，并且还探讨了 CD40/CD40L 系统在 PSE 中的作用。在 410 例缺血性卒中（IS）患者和 389 例 PSE 患者中进行了病例对照研究。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。通过实时 PCR 和 Western blot 分别测定 CD40 mRNA 和蛋白水平。使用 ELISA 试剂盒检测血浆 sCD40L 水平。PSE 患者中的 T 等位基因频率明显高于 IS 患者（P<0.05）。与健康对照组和 IS 患者相比，PSE 患者的血浆 sCD40L 水平明显更高（P<0.01，P<0.05）。与健康对照组和 IS 患者相比，PSE 患者的外周血单个核细胞（PBMCs）中 CD40 mRNA 和蛋白表达明显更高（P<0.01，P<0.05）。在 PSE 患者中，T 等位基因携带者的血浆 sCD40L 水平升高，CD40 mRNA 表达增加。我们的研究表明，CD40-1C/T 多态性的 T 等位基因可能与 PSE 易感性相关。CD40/CD40L 系统参与 PSE 的发生过程。
