利用新分离的肺炎克雷伯菌 J2B 从甘油生产 1,3-丙二醇。
Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B.
机构信息
School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
出版信息
Bioresour Technol. 2014 May;159:223-31. doi: 10.1016/j.biortech.2014.01.126. Epub 2014 Feb 7.
Recently, novel Klebsiella pneumoniae J2B, which grows rapidly on glycerol as the carbon source without forming pathogenic and sticky lipopolysaccharides, was isolated. Current study examined the ability of K. pneumoniae J2B to produce 1,3-propanediol (PDO) from glycerol. To this end, a deletion mutant for lactate formation was constructed. The ldhA mutant strain produced negligible lactate but more 2,3-butanediol (BDO). When K. pneumoniae ΔldhA was cultivated in glycerol fed-batch mode, the PDO titer of 58.0 g/L with a yield of 0.35 g/g and an overall volumetric productivity of 1.3g/L/h were obtained. BDO was the main byproduct (26.6g/L). Less than 10 g/L of the other metabolites was produced. As PDO and other metabolites accumulated, the rate of PDO production decreased significantly due mainly to the toxic effects of these metabolites. This study highlights the potential of newly isolated K. pneumoniae J2B for the production of PDO from glycerol.
最近，分离到了一种新型肺炎克雷伯氏菌 J2B，它可以在甘油作为碳源的情况下快速生长，而不会形成致病的粘性脂多糖。本研究考察了肺炎克雷伯氏菌 J2B 利用甘油生产 1,3-丙二醇（PDO）的能力。为此，构建了一个用于乳酸形成的缺失突变体。该 ldhA 突变株几乎不产生乳酸，但会产生更多的 2,3-丁二醇（BDO）。当肺炎克雷伯氏菌 ΔldhA 在甘油分批补料培养中培养时，可获得 58.0 g/L 的 PDO 产率，产率为 0.35 g/g，总体体积生产率为 1.3 g/L/h。BDO 是主要的副产物（26.6 g/L）。生成的其他代谢物少于 10 g/L。随着 PDO 和其他代谢物的积累，由于这些代谢物的毒性作用，PDO 的生产速率显著下降。本研究强调了新型肺炎克雷伯氏菌 J2B 从甘油生产 PDO 的潜力。
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