Gómez-Pérez María Luz, Plaza-Bolaños Patricia, Romero-González Roberto, Martínez Vidal José Luis, Garrido Frenich Antonia
J Am Soc Mass Spectrom. 2014 May;25(5):899-902. doi: 10.1007/s13361-014-0849-4.
A method based on gas chromatography-atmospheric pressure chemical ionization-mass spectrometry (GC-APCI-MS) has been developed for the analysis of pesticides in meat by using quadrupole-time of flight mass spectrometry (QTOF-MS). Ionization and MS conditions were studied for 71 compounds, although only 51 showed acceptable performance. The protonated form of the analytes was mainly found ([M + H]⁺), although some compounds generated the molecular ion (M⁺(•)). A fast and generic extraction procedure was applied in sample pretreatment. The analytical method was suitable for qualitative analysis, and it was also evaluated for quantitative analysis, obtaining adequate recovery and precision values for most of the studied analytes at two concentration levels (50 and 150 μg/kg). Several operational drawbacks were found with this instrument, such as slow stabilization and moderate sensitivity, although the fast switching between LC and GC allows the increase of its applicability.
已开发出一种基于气相色谱-大气压化学电离-质谱联用（GC-APCI-MS）的方法，通过四极杆飞行时间质谱（QTOF-MS）分析肉类中的农药。对71种化合物的电离和质谱条件进行了研究，尽管只有51种表现出可接受的性能。分析物的质子化形式主要被发现（[M + H]⁺），尽管一些化合物产生了分子离子（M⁺(•)）。在样品预处理中采用了快速通用的提取程序。该分析方法适用于定性分析，还对定量分析进行了评估，在两个浓度水平（50和150μg/kg）下，大多数研究的分析物获得了足够的回收率和精密度值。该仪器存在一些操作上的缺点，如稳定缓慢和灵敏度适中，尽管液相色谱和气相色谱之间的快速切换增加了其适用性。
