Maryanski J L, Abastado J P, MacDonald H R, Kourilsky P
Ludwig Institute for Cancer Research, Epalinges, Switzerland.
Eur J Immunol. 1989 Jan;19(1):193-6. doi: 10.1002/eji.1830190131.
To identify residues on class I major histocompatibility complex (MHC) antigens that are important in T cell recognition, we analyzed a series of 11 intradomain recombinant mouse MHC (H-2) molecules in which N-terminal H-2Kd segments of varying lengths are followed by H-2Dd segments. Lysis of L cell transfectant target cells by a series of alloreactive cytolytic T cell (CTL) clones specific for Kd or for Dd revealed several regions that contain residues critical for specific recognition. These residues map within the presumed antigen-binding site of the MHC molecule. Of particular interest was the finding that the two regions identified as important for Kd allorecognition match those that influence the recognition of synthetic peptide antigens by Kd-restricted CTL.
为了鉴定Ⅰ类主要组织相容性复合体（MHC）抗原上对T细胞识别很重要的残基，我们分析了一系列11个结构域内重组小鼠MHC（H-2）分子，其中不同长度的N端H-2Kd片段后接H-2Dd片段。一系列对Kd或Dd特异的同种异体反应性细胞毒性T细胞（CTL）克隆对L细胞转染靶细胞的裂解揭示了几个含有对特异性识别至关重要的残基的区域。这些残基定位在MHC分子假定的抗原结合位点内。特别令人感兴趣的是，被确定对Kd同种异体识别重要的两个区域与那些影响Kd限制性CTL对合成肽抗原识别的区域相匹配。
