Kehrel B, Kokott R, Stenzinger W, Balleisen L
Institute for Arteriosclerosis Research, University of Münster, FRG.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1988;115(4):425-9.
The application of the lectin-avidin-biotin-peroxidase (LABP) technique for detecting platelet glycoprotein abnormalities in thrombocytopathias is described. Platelet proteins from patients with Glanzmann's thrombasthenia or Bernard-Soulier syndrome were separated by two-dimensional O'Farrell gel electrophoresis, stained with silver or electroblotted onto nitrocellulose sheets. Nitro-cellulose blots were stained utilizing the LABP technique. The absence or severe reduction of glyco-proteins IIb and IIIa and fibrinogen in the platelet protein pattern of patients with thrombasthenia as well as the absence or marked reduction of glycoproteins Ib and V in the platelet protein pattern of a patient with Bernard-Soulier syndrome were clearly demonstrated.
描述了凝集素-抗生物素蛋白-生物素-过氧化物酶（LABP）技术在检测血小板病中血小板糖蛋白异常方面的应用。来自Glanzmann血小板无力症或Bernard-Soulier综合征患者的血小板蛋白通过二维O'Farrell凝胶电泳进行分离，用银染色或电印迹到硝酸纤维素膜上。利用LABP技术对硝酸纤维素印迹进行染色。血小板无力症患者的血小板蛋白图谱中糖蛋白IIb和IIIa以及纤维蛋白原的缺失或严重减少，以及Bernard-Soulier综合征患者的血小板蛋白图谱中糖蛋白Ib和V的缺失或显著减少均得到了明确证实。
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