Tutusaus Joan, López-Gatius Fernando, Serrano Beatriz, Monleón Eva, Badiola Juan José, Garcia-Ispierto Irina
University of Lleida Agrotecnio Centre Av. Alcalde Rovira Roure 191 25198 Lleida Spain.
University of Zaragoza Centre for Research on Transmissible Spongiform Encephalopathies and Emergent Diseases Zaragoza Spain.
Acta Vet Hung. 2014 Jun;62(2):145-54. doi: 10.1556/AVet.2014.005.
This study sought to assess the effects of an inactivated phase I vaccine against Coxiella burnetii at the start of the third trimester of gestation on serological profiles, bacterial shedding patterns and subsequent reproductive performance in dairy cows. Cows were randomly assigned to a control (n = 78) or a vaccinated (n = 78) group on days 171-177 of gestation. Samples of placenta and colostrums at parturition, vaginal fluid, faeces, milk (PCR identification) and blood (anti-C. burnetii antibody detection) were obtained on the day of treatment and on days 91-97 post partum, and also on parturition day and weekly on days 1-7, 8-14, 15-21, 22-28 and 29-35 post partum in a subset of 70 animals. By Kaplan-Meier survival analysis, no significant effect of vaccination was detected on any of the reproductive variables studied. According to the odds ratio, C. burnetii shedding on days 171-177 of gestation was highly correlated with seropositivity against C. burnetii (OR = 9.1), while vaccination was not linked to reduced shedding of the bacterium. In shedders compared to others, the likelihood of pregnancy to first AI decreased and increased by factors of 0.26 and 16.1 on days 1-35 and 91-97 post partum, respectively. In conclusion, when administered at the start of the third trimester of pregnancy, the inactivated C. burnetii phase I vaccine failed to reduce bacterial shedding.
本研究旨在评估妊娠晚期（妊娠第三个月开始）接种的针对伯氏考克斯氏体的灭活I期疫苗对奶牛血清学特征、细菌排泄模式及后续繁殖性能的影响。在妊娠第171 - 177天，奶牛被随机分为对照组（n = 78）和接种组（n = 78）。在治疗当天、产后91 - 97天、分娩当天以及在70头动物的子集中于产后第1 - 7天、8 - 14天、15 - 21天、22 - 28天和29 - 35天每周采集胎盘和初乳、阴道分泌物、粪便、牛奶（PCR鉴定）及血液（抗伯氏考克斯氏体抗体检测）样本。通过Kaplan - Meier生存分析，未检测到疫苗接种对所研究的任何繁殖变量有显著影响。根据优势比，妊娠第171 - 177天伯氏考克斯氏体排泄与抗伯氏考克斯氏体血清阳性高度相关（OR = 9.1），而疫苗接种与细菌排泄减少无关。与其他动物相比，在产后第1 - 35天和91 - 97天，排泄细菌的动物首次人工授精受孕的可能性分别降低和增加了0.26倍和16.1倍。总之，在妊娠晚期开始接种时，灭活的伯氏考克斯氏体I期疫苗未能减少细菌排泄。
