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用哈格曼因子片段(F XIIf)激活法测定人血浆中的激肽释放酶原(KKN)。

Determination of kallikreinogen (KKN) in human plasma by activation with Hageman factor fragment (F XIIf).

作者信息

Lehmann B, Taucher M, Kühne H, Scheuch D W

机构信息

Institute of Pathological Biochemistry, Medical Academy Carl Gustav Carus Dresden.

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1988;115(4):569-72.

PMID:2465974
Abstract

On the basis of activation of KKN by F XIIf an assay for determination of KKN levels was developed. The esterolytic activity of the activation product PK was measured with BAEE as substrate. After maximal activation of normal human plasma the esterolytic activity amounts to 16.82 +/- 2.03 mukat/l plasma (n = 25). The within run precision and day to day precision characterized by the variation coefficient were 2.5 and 4.9, respectively.

摘要

基于FⅫ对KKN的激活作用,开发了一种测定KKN水平的检测方法。以BAEE为底物测定激活产物PK的酯解活性。正常人血浆最大激活后,酯解活性为16.82±2.03微卡特/升血浆(n = 25)。以变异系数表征的批内精密度和日间精密度分别为2.5和4.9。

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