Lu Xianghong, Zhang Qiuhong, Lu Meizhen, Dou Xiao, Huang Chenlei, Jia Junqian, Ji Jianbing
Sheng Wu Gong Cheng Xue Bao. 2013 Dec;29(12):1865-9.
Nitrogen source is one of the important factors that affect the microalgae growth and lipid accumulation. We studied the effects of various nitrogen sources (i.e. NaNO3, CO(NH2)2, NH4Cl and CH3COONH4) and amount on the growth density, lipid yield, and eicosapentaenoic acid (EPA) content of Nannochloropsis oculata by single factor experimental method. The results show that N. oculata preferred NH4+ as nitrogen source rather than NO3- and CO(NH2)2. NH4+ could promote the growth and lipid accumulation of N. oculata. With the increase of nitrogen concentration, the biomass and the content of polyunsaturated fatty acid (PUFA) increased, but the content of lipid decreased. CH3COONH4 was the most suitable for growth, accumulation of lipid and EPA of N. oculata among the four investigated nitrogen sources. The optimal concentration was 5.29 mmol/L.
氮源是影响微藻生长和脂质积累的重要因素之一。我们采用单因素实验方法研究了不同氮源（即硝酸钠、尿素、氯化铵和醋酸铵）及其用量对眼点拟微球藻生长密度、脂质产量和二十碳五烯酸（EPA）含量的影响。结果表明，眼点拟微球藻更倾向于以铵根离子作为氮源，而非硝酸根离子和尿素。铵根离子能够促进眼点拟微球藻的生长和脂质积累。随着氮浓度的增加，生物量和多不饱和脂肪酸（PUFA）含量增加，但脂质含量降低。在所研究的四种氮源中，醋酸铵最适合眼点拟微球藻的生长、脂质积累和EPA积累。最佳浓度为5.29 mmol/L。
