Department of Chemistry, Graduate School of Science, Kyoto University , Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Nano Lett. 2014 May 14;14(5):2286-92. doi: 10.1021/nl4044949. Epub 2014 Apr 4.
We report the use of atomic force microscopy (AFM) to study Sox2-Pax6 complex formation on the regulatory DNA element at a single molecule level. Using an origami DNA scaffold containing two DNA strands with different levels of tensile force, we confirmed that DNA bending is necessary for Sox2 binding. We also demonstrated that two transcription factors bind cooperatively by observing the increased occupancy of Sox2-Pax6 on the DNA element compared to that of Sox2 alone.
我们报告了使用原子力显微镜（AFM）在单个分子水平上研究 Sox2-Pax6 复合物在调节 DNA 元件上的形成。使用含有两条具有不同拉伸力水平的 DNA 链的折纸 DNA 支架，我们证实了 DNA 弯曲对于 Sox2 结合是必要的。我们还通过观察 Sox2-Pax6 在 DNA 元件上的占有率相对于 Sox2 单独结合的占有率增加，证明了两个转录因子通过合作结合。
