Lin Caiyu, Wang Li, Wang Hong, Fang Shengtao, Zhang Quanbo, Yang Liuqi, Guo Huijie, Lin Ping, Zhang Jie, Wang Xiujie
a Laboratory of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital , West China Medical School, Sichuan University , Chengdu , China.
Nutr Cancer. 2014;66(4):613-24. doi: 10.1080/01635581.2014.894094. Epub 2014 Mar 24.
Lithocarpus polystachyus leaves have been used as tea beverage and folk medicine for healthy care in the Southwest of China. The purpose of this study is to investigate the anticancer activity of Lithocarpus polystachyus Rehd leaf aqueous extract (LPAE) and to explore the possible mechanism of its activity. Growth inhibition effects of LPAE breast cancer were tested in vitro and in vivo. The possible mechanism of its activity was analyzed with cell biological and molecular biological assays. After LPAE treatment, the proliferation and colony formation of cancer cells decreased; apoptotic cells increased; DNA fragmentations were evident; mRNA and protein expressions of PPARγ, Bax, and caspase-3 genes increased and expressions of cyclin D1 and Bcl-2 genes decreased; in vivo experiment, LPAE inhibited human beast cancer growth. The findings in this experimental study suggested that LPAE has potential cytotoxic and apoptotic effects on human breast cancer cells in vitro and inhibits the cancer growth in vivo, and its mechanism of activity might be associated with apoptosis induction of cancer cells through upregulation of the mRNA and protein expressions of PPARγ, Bax, and capase-3 genes and downregulation of the expressions of cyclin D1 and Bcl-2 genes.
石栎叶在中国西南部地区一直被用作茶饮料和用于保健的民间药物。本研究旨在探讨石栎叶水提取物（LPAE）的抗癌活性，并探究其活性的可能机制。在体外和体内测试了LPAE对乳腺癌的生长抑制作用。通过细胞生物学和分子生物学实验分析其活性的可能机制。LPAE处理后，癌细胞的增殖和集落形成减少；凋亡细胞增加；DNA片段化明显；PPARγ、Bax和caspase-3基因的mRNA和蛋白表达增加，而细胞周期蛋白D1和Bcl-2基因的表达减少；在体内实验中，LPAE抑制人乳腺癌生长。本实验研究结果表明，LPAE在体外对人乳腺癌细胞具有潜在的细胞毒性和凋亡作用，并在体内抑制肿瘤生长，其活性机制可能与通过上调PPARγ、Bax和capase-3基因的mRNA和蛋白表达以及下调细胞周期蛋白D1和Bcl-2基因的表达来诱导癌细胞凋亡有关。
