Department of Oral and Maxillofacial Prosthodontics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
J Appl Microbiol. 2014 Jun;116(6):1531-42. doi: 10.1111/jam.12483. Epub 2014 Mar 24.
The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis).
Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μg ml(-1)) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μg ml(-1)) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C. albicans biofilms exposed to a low concentration of DNA (<1·0 μg ml(-1)).
These findings demonstrated that extracellular DNA (eDNA) plays a crucial role in Candida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C. albicans biofilm development.
A novel therapy targeting eDNA may be applicable for Candida infection to decrease biofilm formation and hyphal formation.
本研究旨在探讨从白色念珠菌和肺炎相关病原体铜绿假单胞菌和金黄色葡萄球菌中提取的基因组 DNA 对 3 种念珠菌（白色念珠菌、光滑念珠菌和热带念珠菌）体外生物膜形成和形态变化的影响。
采用结晶紫法和菌落形成单位计数评估生物膜形成。通过扫描电子显微镜和荧光显微镜评估生物膜的形态特征。低浓度（<1.0μg/ml）的 DNA 显著增加了所有 3 种念珠菌的生物膜质量。相反，高浓度（10μg/ml）的 DNA 降低了生物膜质量。有趣的是，在暴露于低浓度 DNA（<1.0μg/ml）的白色念珠菌生物膜中观察到密集的酵母细胞中菌丝的形成。
这些发现表明细胞外 DNA（eDNA）在念珠菌生物膜形成中起关键作用，并表明 eDNA 可能在白色念珠菌生物膜发育过程中诱导从酵母到菌丝生长形态的形态转变。
针对 eDNA 的新型治疗方法可能适用于念珠菌感染，以减少生物膜形成和菌丝形成。
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