Köller U, Haas O A, Kornmüller R, Majdic O, Gadner H, Knapp W
Institut für Klinische Chemie und Laboratoriumsdiagnostik, Universität Wien.
Wien Klin Wochenschr. 1989 Feb 3;101(3):111-7.
Cytogenetic analysis and immunological phenotyping are well established methods for characterizing leukaemic cell populations. The simultaneous evaluation of cell phenotype and karyotype has, however, been established only recently. We present immunocytochemical methods utilizing horseradish peroxidase and alkaline phosphatase monoclonal anti-alkaline phosphatase staining procedures, as well as modified immunoflurescence methods with fluorescein- and rhodamine-conjugated antibodies. Chromosomes were either stained with Giemsa and "stains-all" (immunocytochemical techniques) or chromomycin and distamycin A/DAPI for R- and C-banding (immunofluorescence techniques), respectively. The advantages and disadvantages of these methods are compared and provide representative examples of the various preparations. These methods will contribute to defining the phenotype of cytogenically-normal cells in leukaemias and to assigning different karyotypes to heterogenous cell populations, as well as supporting the search for analysis of residual blast cells.
细胞遗传学分析和免疫表型分析是表征白血病细胞群体的成熟方法。然而，细胞表型和核型的同时评估直到最近才得以确立。我们介绍了利用辣根过氧化物酶和碱性磷酸酶单克隆抗碱性磷酸酶染色程序的免疫细胞化学方法，以及使用荧光素和罗丹明偶联抗体的改良免疫荧光方法。染色体分别用吉姆萨和“全染剂”（免疫细胞化学技术）染色，或用色霉素和放线菌素A/4',6-二脒基-2-苯基吲哚进行R带和C带染色（免疫荧光技术）。比较了这些方法的优缺点，并提供了各种制备方法的代表性实例。这些方法将有助于确定白血病中细胞遗传学正常细胞的表型，将不同的核型分配给异质性细胞群体，并支持对残留原始细胞的分析研究。
