Sarkar Dibyo, Gunda Naga Siva Kumar, Jamal Iqbal, Mitra Sushanta K
Department of Mechanical Engineering, University of Alberta, Edmonton, T6G 2G8, Canada.
Biomed Microdevices. 2014 Aug;16(4):509-20. doi: 10.1007/s10544-014-9853-5.
In this work, we have demonstrated an efficient optical immunoassay technique for the detection of a food-borne pathogen, Listeria monocytogenes, using a Mach-Zehnder Interferometer (MZI) configuration. We have investigated ten different MZI configurations with angular and Sbend Y-junction geometries. An efficient Hydrofluoric acid (HF) based technique was used for rapid and specific binding of L. monocytogenes to the sensor arm of the MZI biosensor. The MZI biosensor was able to detect L. monocytogenes at concentrations of the order of 10(5) CFU/ml, which is lower than the infection dose for healthy human beings. SEM analysis and light intensity measurements showed the biosensor is highly selective to L. monocytogenes over other microbial species (such as Escherichia coli). Finally, a novel calibration scheme of the MZI biosensor was developed from experimental data that can be used for determining unknown concentrations of L. monocytogenes.
在这项工作中，我们展示了一种高效的光学免疫分析技术，用于检测食源性病原体单核细胞增生李斯特菌，该技术采用马赫-曾德尔干涉仪（MZI）配置。我们研究了十种具有角形和S形弯曲Y型结几何结构的不同MZI配置。采用了一种基于氢氟酸（HF）的高效技术，使单核细胞增生李斯特菌能够快速、特异性地结合到MZI生物传感器的传感臂上。该MZI生物传感器能够检测浓度约为10⁵CFU/ml的单核细胞增生李斯特菌，这一浓度低于健康人的感染剂量。扫描电子显微镜（SEM）分析和光强测量表明，该生物传感器对单核细胞增生李斯特菌的选择性高于其他微生物物种（如大肠杆菌）。最后，根据实验数据开发了一种MZI生物传感器的新型校准方案，可用于确定未知浓度的单核细胞增生李斯特菌。
