Hirasé Y, Valauri F A, Buncke H J
Department of Plastic Surgery, Jikei University School of Medicine, Tokyo, Japan.
Ann Plast Surg. 1988 Oct;21(4):342-7. doi: 10.1097/00000637-198810000-00008.
In preparation for this study in rabbits, preliminary trials were conducted in rats rather than rabbits because they are more economical and are believed to develop neovascularization more rapidly. Using modifications of techniques described by Duarte, the superficial inferior epigastric vessels were planted between the folded leaves of full-thickness grafts of rat ear cartilage. These preparations showed signs of neovascularization and new cartilage formation adjacent to the pedicle when harvested from the groin three weeks later. Encouraged by the success of this preliminary investigation in the rat, we moved on to the rabbit ear model because it provides a larger area of cartilage to work with. The central vessels of the pinna were dissected out and then placed in a subcutaneous (extraperichondrial) pocket of a random-pattern, distally based, full-thickness ear flap. Three weeks later, the flaps, now neovascularized by their neopedicles of implanted central vessels, were harvested and transferred as free chondrocutaneous free flaps to the donor site of the same preparation created on the contralateral pinna. Modifications of these techniques may be useful where chondral or chondrocutaneous flaps are needed, such as for pinna, nasal alar, or nasal septal reconstructions.
在为这项兔实验做准备时，由于大鼠更经济且据信其血管生成速度更快，所以先在大鼠而非兔身上进行了初步试验。采用对杜阿尔特所描述技术的改进方法，将腹壁下浅血管植入大鼠耳软骨全层移植物的折叠叶片之间。三周后从腹股沟取出这些标本时，在蒂部附近可见血管生成和新软骨形成的迹象。受大鼠初步研究成功的鼓舞，我们转而采用兔耳模型，因为它能提供更大面积的软骨用于操作。将耳廓的中央血管解剖出来，然后置于一个随机模式、远端带蒂的全层耳瓣的皮下（软骨膜外）袋中。三周后，现已通过植入的中央血管新蒂实现血管化的耳瓣被取下，并作为游离软骨皮瓣转移到对侧耳廓上相同制备的供区。这些技术的改进在需要软骨瓣或软骨皮瓣时可能有用，比如用于耳廓、鼻翼或鼻中隔重建。
