在自身免疫性慢性肝炎中，一种人类细胞色素P-450被抗肝肾微粒体抗体识别。
A human cytochrome P-450 is recognized by anti-liver/kidney microsome antibodies in autoimmune chronic hepatitis.
作者信息
Kiffel L, Loeper J, Homberg J C, Leroux J P
机构信息
INSERM U 75, CHU Necker-Enfants Malades, Paris.
出版信息
Biochem Biophys Res Commun. 1989 Feb 28;159(1):283-9. doi: 10.1016/0006-291x(89)92435-2.
1- Anti-liver/kidney microsome autoantibodies type 1 (anti-LKM1), observed in some children with chronic active hepatitis, were used to isolate their antigen in human liver microsomes. A protein, called P-LKM1 was thus purified. This protein was recognized by a rabbit antiserum directed against the related human cytochromes P-450 bufI and P-450 bufII. 2- A human liver microsomal protein immunoprecipitated with anti-LKM1 sera was also recognized by anti cytochromes P-450 bufI/II antibodies. 3- Anti-LKM1 antibodies potently inhibited microsomal bufuralol 1'-hydroxylation. These results displayed the possible identity between cytochrome P-450 bufI/II and LKM1 antigen.
- 在一些慢性活动性肝炎患儿中观察到的1型抗肝肾微粒体自身抗体（抗-LKM1），被用于在人肝微粒体中分离其抗原。由此纯化出一种名为P-LKM1的蛋白质。该蛋白质能被针对相关人细胞色素P-450 bufI和P-450 bufII的兔抗血清识别。2. 用抗-LKM1血清免疫沉淀的一种人肝微粒体蛋白也能被抗细胞色素P-450 bufI/II抗体识别。3. 抗-LKM1抗体强烈抑制微粒体布非洛尔1'-羟化作用。这些结果表明细胞色素P-450 bufI/II与LKM1抗原之间可能存在同一性。
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