MacLean D B, Bennett B, Morris M, Wheeler F B
Division of Endocrinology, Brown University, Rhode Island Hospital, Providence 02903.
Brain Res. 1989 Jan 30;478(2):349-55. doi: 10.1016/0006-8993(89)91515-1.
Nodose (inferior vagal sensory) ganglia were removed from neonatal rats, enzymatically dispersed using neutral protease, and maintained on previously dispersed rat atriacytes. After 7-10 days in culture, calcitonin gene-related peptide (CGRP) was present in 1-3 times the molar amount of substance P (SP). The content of SP was doubled by the addition of nerve growth factor (NGF) whereas CGRP was significantly less increased by 50% or less. The addition of forskolin increased SP and CGRP levels in cultures with or without NGF by 60-80 percent. Phorbol ester (PMA) did not alter SP content but significantly raised CGRP content by 40% in NGF supplemented cultures (P less than 0.001). Corticosterone, 0.01-0.1 microM, reduced SP content by 30% independently of NGF but had no effect on CGRP. These studies demonstrate that SP in vagal sensory neurons is more sensitive than CGRP to the effects of NGF or corticosterone. Both peptides are up-regulated by presumed increases in intracellular cyclic AMP, while CGRP (or CGRP neurons) may be independently regulated by protein kinase C.
从新生大鼠中取出结状（迷走神经下感觉）神经节，用中性蛋白酶进行酶解分散，并培养于先前已分散的大鼠心房肌细胞上。培养7 - 10天后，降钙素基因相关肽（CGRP）的摩尔量是P物质（SP）的1 - 3倍。添加神经生长因子（NGF）后，SP的含量增加了一倍，而CGRP的增加量明显较少，增幅在50%或更低。添加福斯高林后，无论有无NGF，培养物中SP和CGRP的水平均增加了60 - 80%。佛波酯（PMA）不改变SP的含量，但在添加NGF的培养物中使CGRP的含量显著提高了40%（P < 0.001）。0.01 - 0.1微摩尔的皮质酮可使SP的含量独立于NGF降低30%，但对CGRP无影响。这些研究表明，迷走神经感觉神经元中的SP对NGF或皮质酮的作用比CGRP更敏感。两种肽都可能因细胞内环磷酸腺苷的假定增加而上调，而CGRP（或CGRP神经元）可能受蛋白激酶C的独立调节。
