Helke C J, Niederer A J
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
Synapse. 1990;5(2):144-51. doi: 10.1002/syn.890050209.
Visceral afferent neurons of the nodose and petrosal ganglia are immunoreactive (ir) for many neurotransmitters [e.g., substance P (SP), neurokinin A (NKA), calcitonin gene-related peptide (CGRP), and dopamine (tyrosine hydroxylase-ir; TH)]. Coexistence of SP-ir with NKA-, CGRP-, or TH-ir was studied in individual neurons of the rat ganglia using fluorescence immunocytochemistry. SP- and NKA-ir were present in equal numbers of cells and were consistently colocalized. SP- and CGRP-ir were found to be similarly distributed in scattered cells, concentrated mostly in the rostral pole of the nodose ganglion and in the petrosal ganglion. SP-ir completely coexisted with CGRP-ir. However, there was at least twice the number of CGRP-ir neurons as SP-ir neurons, and thus CGRP-ir neurons that did not contain SP-ir were also present. In contrast, SP- and TH-ir had different distributions in both the nodose and the petrosal ganglia. SP-ir was located in the more rostral regions of both the nodose and petrosal ganglia, whereas TH-ir was detected throughout the entire nodose ganglion and only in the most caudal region of the petrosal ganglion. There was no coexistence of SP- and TH-ir. These data demonstrate the differential localization and coexistence of putative transmitters in visceral sensory neurons in the nodose and petrosal ganglia.
结状神经节和岩神经节的内脏传入神经元对多种神经递质具有免疫反应性（ir）[例如，P物质（SP）、神经激肽A（NKA）、降钙素基因相关肽（CGRP）和多巴胺（酪氨酸羟化酶免疫反应性；TH）]。利用荧光免疫细胞化学技术，在大鼠神经节的单个神经元中研究了SP免疫反应性与NKA、CGRP或TH免疫反应性的共存情况。SP免疫反应性和NKA免疫反应性存在于数量相等的细胞中，并且始终共定位。发现SP免疫反应性和CGRP免疫反应性在散在细胞中的分布相似，主要集中在结状神经节的头端和岩神经节中。SP免疫反应性与CGRP免疫反应性完全共存。然而，CGRP免疫反应性神经元的数量至少是SP免疫反应性神经元的两倍，因此也存在不含有SP免疫反应性的CGRP免疫反应性神经元。相比之下，SP免疫反应性和TH免疫反应性在结状神经节和岩神经节中的分布不同。SP免疫反应性位于结状神经节和岩神经节的更头端区域，而TH免疫反应性在整个结状神经节中都能检测到，仅在岩神经节的最尾端区域能检测到。SP免疫反应性和TH免疫反应性不存在共存情况。这些数据证明了结状神经节和岩神经节中内脏感觉神经元中假定递质的差异定位和共存情况。
