Scriba G K, Borchardt R T, Zirrolli J A, Fennessey P V
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
J Chromatogr. 1988 Dec 9;433:31-40. doi: 10.1016/s0378-4347(00)80582-4.
The sensitivity of catecholamines using selected-ion monitoring gas chromatography-mass spectrometry (SIM GC-MS) has been enhanced by employing a simple clean-up step using Sephadex G-10 columns. This procedure allows for the detection of extremely small amounts (1-10 pg) of biogenic amines (e.g., epinine) which corresponds to a 25-fold increase in detection limits compared to electron ionization GC-MS or high-performance liquid chromatography with electrochemical detection (HPLC-ED). The SIM GC-MS assay was used to monitor the dopamine-beta-hydroxylase-mediated conversion of epinine d6 to epinephrine d6 in rat hypothalamus and brainstem in vitro and the results were compared to those obtained by HPLC-ED.
通过使用葡聚糖G-10柱进行简单的净化步骤，采用选择离子监测气相色谱-质谱联用仪（SIM GC-MS）测定儿茶酚胺的灵敏度得到了提高。该方法能够检测极少量（1-10皮克）的生物胺（如去甲肾上腺素），与电子电离气相色谱-质谱联用仪或电化学检测高效液相色谱法（HPLC-ED）相比，检测限提高了25倍。利用SIM GC-MS分析法监测大鼠下丘脑和脑干中多巴胺-β-羟化酶介导的去甲肾上腺素d6向肾上腺素d6的体外转化，并将结果与HPLC-ED法所得结果进行比较。
