Waites G T, James R F, Bell S C
Department of Obstetrics and Gynaecology, University of Leicester, Leicester Royal Infirmary.
J Endocrinol. 1989 Feb;120(2):351-7. doi: 10.1677/joe.0.1200351.
We have previously shown that pregnancy-associated endometrial alpha 1-globulin, a small molecular weight insulin-like growth factor-binding protein (IGF-BP), is quantitatively the major secretory protein product of the decidualized endometrium during human pregnancy. In the present study, employing monoclonal antibodies raised against this protein in an immunohistological technique, the cellular localization of the protein has been examined in the decidua and placenta during pregnancy. During the first trimester the protein was principally associated with the decidual cell in the decidualized decidua compacta region of the endometrium with both cytoplasmic and extracellular matrix-associated staining patterns being detected. No extensive staining was observed in the placenta. At term the protein was localized in similar cells in the placental bed and endometrium associated with the amniochorion but not in the placenta. These studies suggest that the decidual cell represents the major source of IGF-BP during pregnancy and have relevance to the origin of amniotic fluid IGF-BP and the paracrine role of the decidual cell in the control of trophoblast growth.
我们之前已经表明，妊娠相关子宫内膜α1球蛋白，一种小分子量胰岛素样生长因子结合蛋白（IGF - BP），在数量上是人类妊娠期间蜕膜化子宫内膜的主要分泌蛋白产物。在本研究中，采用针对该蛋白产生的单克隆抗体，运用免疫组织学技术，研究了该蛋白在妊娠期间蜕膜和胎盘中的细胞定位。在妊娠早期，该蛋白主要与子宫内膜蜕膜化致密层区域的蜕膜细胞相关，检测到细胞质和细胞外基质相关的染色模式。在胎盘中未观察到广泛染色。足月时，该蛋白定位于胎盘床和与羊膜绒毛膜相关的子宫内膜中的类似细胞，但不在胎盘中。这些研究表明，蜕膜细胞是妊娠期间IGF - BP的主要来源，并且与羊水IGF - BP的起源以及蜕膜细胞在控制滋养层生长中的旁分泌作用相关。
