[采用超高效液相色谱法测定婴幼儿配方奶粉中的叶黄素]

[Determination of lutein in infant formula milk powder using ultra-high performance liquid chromatography].

作者信息

Wang Lin, Huang Junrong, Zhang Li, Feng Feng, Ling Yun, Chu Xiaogang, Li Hongliang

出版信息

Se Pu. 2013 Dec;31(12):1228-31. doi: 10.3724/sp.j.1123.2013.10007.

DOI:10.3724/sp.j.1123.2013.10007

Abstract

An ultra-high performance liquid chromatography (U-HPLC) method for the determination of lutein in the infant formula milk powder was developed. The sample was extracted with acetone and defatted using freezing centrifugation method. The U-HPLC separation was achieved using a YMC Carotenoid C30 column (150 mm x 4.6 mm, 3 microm) with the mixture of methanol/methyl tert-butyl ether (70: 30, v/v) as the mobile phase under isocratic elution. The flow rate was 0.5 mL/min and the column oven temperature was 25 degrees C. The injection volume was 5 microL. It was detected on a photodiode array detector at a wavelength of 445 nm. The results showed that the linear range was 20-500 microg/L (r = 0.9999), and the limit of quantification was 20 microg/L. The mean recoveries of lutein varied from 97.9% to 104.4% spiked at 50, 250 and 2,000 microg/kg. The established method is simple, accurate and sensitive for the rapid determination of lutein in infant formula milk powder.

摘要

建立了一种用于测定婴儿配方奶粉中叶黄素的超高效液相色谱（U-HPLC）方法。样品用丙酮提取，并采用冷冻离心法脱脂。使用YMC类胡萝卜素C30柱（150 mm×4.6 mm，3μm），以甲醇/甲基叔丁基醚（70:30，v/v）的混合物作为流动相，在等度洗脱条件下进行U-HPLC分离。流速为0.5 mL/min，柱温箱温度为25℃。进样体积为5μL。在光电二极管阵列检测器上于445 nm波长处进行检测。结果表明，线性范围为20 - 500μg/L（r = 0.9999），定量限为20μg/L。在50、250和2000μg/kg加标水平下，叶黄素的平均回收率在97.9%至104.4%之间。所建立的方法简便、准确、灵敏，可用于快速测定婴儿配方奶粉中的叶黄素。