He Qiaosang, Liu Minfang, Huang Liying, Yang Yuanyuan, Liao Shangfu
Zhejiang Test Academy of Quality and Technical Supervision, Hangzhou 310013, China.
Se Pu. 2008 Nov;26(6):752-4.
The analytical method of melamine in milk and milk powder by high performance liquid chromatography (HPLC) was established. The sample was purified by a cation exchange solid-phase extraction column, separated on a Diamonsil C18 column (4.6 mm x 200 mm, 5 microm) with acetonitrile-0.01 mol/L sodium heptanesulfonate (pH 3.3) (10:90, v/v) as mobile phase at a flow rate of 1.0 mL/min, and determined by a photodiode array detector. A linear calibration curve was obtained in the concentration range of 1 - 500 mg/L. The qualification limit was 0.2 mg/kg, and the quantification limit was 1 mg/kg. The recovery was 81.4% -83.7%.
建立了高效液相色谱法（HPLC）测定牛奶和奶粉中三聚氰胺的分析方法。样品经阳离子交换固相萃取柱净化，在Diamonsil C18柱（4.6 mm×200 mm，5μm）上，以乙腈-0.01 mol/L庚烷磺酸钠（pH 3.3）（10:90，v/v）为流动相，流速为1.0 mL/min进行分离，采用光电二极管阵列检测器测定。在1 - 500 mg/L浓度范围内获得线性校准曲线。定性限为0.2 mg/kg，定量限为1 mg/kg。回收率为81.4% - 83.7%。
