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筛选作为凝集素和胰蛋白酶抑制剂来源的卡廷加植物。

Screening of Caatinga plants as sources of lectins and trypsin inhibitors.

作者信息

Arcoverde José Hélton Vasconcelos, Carvalho Aline de Souza, Neves Fernanda Pacífico de Almeida, Dionízio Bianca Paiva, Pontual Emmanuel Viana, Paiva Patrícia Maria Guedes, Napoleão Thiago Henrique, Correia Maria Tereza dos Santos, da Silva Márcia Vanusa, Carneiro-da-Cunha Maria das Graças

机构信息

a Departamento de Bioquímica , CCB, Universidade Federal de Pernambuco, Cidade Universitária , 50670-420 Recife , Pernambuco , Brazil.

出版信息

Nat Prod Res. 2014;28(16):1297-301. doi: 10.1080/14786419.2014.900497. Epub 2014 Mar 26.

Abstract

Although it is one of the most threatened areas in the Earth, there are few studies on the biotechnological potential of the Caatinga. This work evaluated 36 extracts from 27 Caatinga plants for lectin and trypsin inhibitor activities. The presence of lectin was detected in 77.7% of samples by haemagglutinating assay. The highest values of specific haemagglutinating activity were found in extracts of leaves from Mimosa lewesii, Bauhinia acuruana and Manilkara rufula and in branches from Myracrodruon urundeuva. Trypsin inhibitor activity was detected in 63.9% of the tested extracts, strong inhibitory effect (>70%) being found in 11 samples. This work demonstrates that Caatinga is a potential source of bioactive plant proteins that can be isolated and studied for several applications. The biochemical prospecting of Caatinga is essential for collection of bioactive principles so as to add conservation value to the region.

摘要

尽管卡廷加是地球上受威胁最严重的地区之一,但对其生物技术潜力的研究却很少。这项工作评估了27种卡廷加植物的36种提取物的凝集素和胰蛋白酶抑制剂活性。通过血细胞凝集试验在77.7%的样品中检测到凝集素的存在。在含羞草、尖叶羊蹄甲和红果人心果的叶片提取物以及乌鲁德乌瓦破布木的树枝提取物中发现了最高的特异性血细胞凝集活性值。在63.9%的测试提取物中检测到胰蛋白酶抑制剂活性,在11个样品中发现了强烈的抑制作用(>70%)。这项工作表明,卡廷加是生物活性植物蛋白的潜在来源,可以分离并用于多种应用研究。对卡廷加进行生化勘探对于收集生物活性成分至关重要,以便为该地区增添保护价值。

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