Varvashevich T N, Nikiforova L S, Bogomazova T V
Lab Delo. 1989(2):61-3.
An iodine-starch method has been developed for the detection of catalase in bacteria and for measuring this enzyme's activity. The method is based on potassium iodide reduction with hydrogen peroxide to form free iodine. The bacteria are layered on starch gel, treated with hydrogen peroxide, and then with potassium iodide. The starch gel stains blue, and light areas emerge round catalase-positive bacteria; the size of these light sites helps assess the enzyme's activity. The method permits estimating the populational heterogeneity of the cultures judging from catalase activities and helps detect the mutants by catalase; it is convenient, simple, and provides reliable information on the taxonomic status of the bacteria.
已开发出一种碘-淀粉法，用于检测细菌中的过氧化氢酶并测定该酶的活性。该方法基于过氧化氢将碘化钾还原形成游离碘。将细菌铺在淀粉凝胶上，用过氧化氢处理，然后用碘化钾处理。淀粉凝胶会染成蓝色，过氧化氢酶阳性细菌周围会出现浅色区域；这些浅色区域的大小有助于评估酶的活性。该方法可以根据过氧化氢酶活性估计培养物的群体异质性，并有助于通过过氧化氢酶检测突变体；它方便、简单，并能提供有关细菌分类地位的可靠信息。
