Avesar Jonathan, Arye Tom Ben, Levenberg Shulamit
Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel 32000.
Lab Chip. 2014 Jul 7;14(13):2161-7. doi: 10.1039/c4lc00013g. Epub 2014 Mar 27.
Here, we review the frontier microfluidic techniques for single cell analysis (SCA), which is important for research of many biological systems. Microfluidics provides high-throughput, high-resolution experiments at low cost and reagent use, making it especially useful for single cell analysis. Recent advancements in the field have made SCA more feasible, improving device throughput and resolution, adding capabilities, and combining different functions to bring forth new assays. Developments in incubation have allowed for long-term cell tracking assays to be performed with single cell resolution. The ability of systems to provide chemical isolation or prolonged growth of adherent cells is also discussed.
在此，我们回顾了用于单细胞分析（SCA）的前沿微流控技术，这对于许多生物系统的研究至关重要。微流控技术能够以低成本和低试剂用量提供高通量、高分辨率的实验，使其在单细胞分析中尤为有用。该领域的最新进展使单细胞分析变得更加可行，提高了设备的通量和分辨率，增加了功能，并将不同功能相结合以产生新的检测方法。孵育方面的进展使得能够以单细胞分辨率进行长期细胞追踪检测。我们还讨论了系统提供化学隔离或使贴壁细胞长期生长的能力。
