Department of Biotechnology, Indian Institute of Technology, Kharagpur, India 721302.
Lab Chip. 2012 Jan 7;12(1):69-73. doi: 10.1039/c1lc20888h. Epub 2011 Nov 10.
Microfluidics based cell culture applications have facilitated the study of cellular dynamics at the single entity level. Yet, long term versions of such applications in a static framework suffer from the fast exhaustion of available oxygen, dissolved in the limited media volume available per cell, within the microconfined environment. In order to circumvent such drawbacks, we have improvised a microfluidic cell culture platform for prolonged sustenance of adherent mammalian cells by formation of an air-liquid interface through functionalizing inner surfaces of a polydimethylsiloxane (PDMS) based microdevice. We have demonstrated an augmented static incubation time for different cell lines using this approach.
基于微流控的细胞培养应用促进了单细胞水平下细胞动力学的研究。然而，在静态框架中长期应用此类应用时，由于微受限环境中每细胞可用的有限培养基体积中溶解的氧气迅速耗尽，因此会受到影响。为了避免这些缺点，我们通过对基于聚二甲基硅氧烷（PDMS）的微器件的内表面进行功能化，构建了一种微流控细胞培养平台，通过形成气-液界面来延长贴壁哺乳动物细胞的长期维持。我们使用这种方法证明了不同细胞系的静态孵育时间得到了延长。
