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溶血磷脂酰胆碱可逆地阻止了多种病毒融合蛋白介导的合胞体形成过程中的孔道扩张。

Lysophosphatidylcholine reversibly arrests pore expansion during syncytium formation mediated by diverse viral fusogens.

机构信息

Department of Microbiology & Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.

出版信息

J Virol. 2014 Jun;88(11):6528-31. doi: 10.1128/JVI.00314-14. Epub 2014 Mar 26.

DOI:10.1128/JVI.00314-14
PMID:24672027
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4093848/
Abstract

Using lysophosphatidylcholine, a curvature-inducing lysolipid, we have isolated a reversible, "stalled pore" phenotype during syncytium formation induced by the p14 fusion-associated small transmembrane (FAST) protein and influenza virus hemagglutinin (HA) fusogens. This is the first evidence that lateral propagation of stable fusion pores leading to syncytiogenesis mediated by diverse viral fusogens is inhibited by promotion of positive membrane curvature in the outer leaflets of the lipid bilayer surrounding intercellular fusion pores.

摘要

使用溶血磷脂酰胆碱，一种诱导曲率的溶血磷脂，我们在由 p14 融合相关小跨膜（FAST）蛋白和流感病毒血凝素（HA）融合蛋白诱导的合胞体形成过程中分离出一种可逆的“停滞孔”表型。这是第一个证据，表明由不同病毒融合蛋白介导的稳定融合孔的横向传播导致合胞体形成被促进脂质双层外层的正膜曲率的方式所抑制。

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Lysophosphatidylcholine reversibly arrests pore expansion during syncytium formation mediated by diverse viral fusogens.溶血磷脂酰胆碱可逆地阻止了多种病毒融合蛋白介导的合胞体形成过程中的孔道扩张。
J Virol. 2014 Jun;88(11):6528-31. doi: 10.1128/JVI.00314-14. Epub 2014 Mar 26.
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本文引用的文献

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Extracellular annexins and dynamin are important for sequential steps in myoblast fusion.细胞外膜联蛋白和动力蛋白对于成肌细胞融合的连续步骤很重要。
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The NS16 protein of aquareovirus-C is a fusion-associated small transmembrane (FAST) protein, and its activity can be enhanced by the nonstructural protein NS26.水生动物呼肠孤病毒-C 的 NS16 蛋白是一种融合相关的小跨膜（FAST）蛋白，其活性可被非结构蛋白 NS26 增强。
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Intracellular curvature-generating proteins in cell-to-cell fusion.细胞间融合中的细胞内曲率生成蛋白。
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The reovirus fusion-associated small transmembrane (FAST) proteins: virus-encoded cellular fusogens.呼肠孤病毒融合相关小跨膜（FAST）蛋白：病毒编码的细胞融合蛋白。
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Broome virus, a new fusogenic Orthoreovirus species isolated from an Australian fruit bat.布鲁姆病毒，一种从澳大利亚果蝠中分离出来的新型融合正呼肠孤病毒。
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Aquareovirus effects syncytiogenesis by using a novel member of the FAST protein family translated from a noncanonical translation start site.水生呼肠孤病毒通过利用从非规范翻译起始位点翻译而来的FAST蛋白家族的一个新成员来影响多核体形成。
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The p14 FAST protein of reptilian reovirus increases vesicular stomatitis virus neuropathogenesis.爬行类呼肠孤病毒的p14 FAST蛋白会增加水疱性口炎病毒的神经致病力。
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Viral membrane fusion.病毒膜融合
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