西咪替丁（一种组胺2型受体拮抗剂）对大鼠全层烧伤所致胃微循环障碍的影响，以此作为手术应激的模型。

Effects of cimetidine, a histamine type 2 receptor antagonist, on microcirculatory disturbance of the stomach induced by full-thickness burn injuries as a model for surgical stress in rats.

作者信息

Ishikawa Hideki, Yoshida Masashi, Wakabayashi Go, Otani Yoshihide, Shimazu Motohide, Kubota Tetsuro, Kumai Koichiro, Aikawa Naoki, Kitajima Masaki

机构信息

Department of Emergency and Critical Care Medicine, Keio University School of Medicine, Tokyo, Japan.

Department of Surgery, Keio University School of Medicine, Tokyo, Japan.

出版信息

Curr Ther Res Clin Exp. 2004 Jul;65(4):373-82. doi: 10.1016/j.curtheres.2004.07.001.

DOI:10.1016/j.curtheres.2004.07.001

PMID:24672092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3964572/

Abstract

BACKGROUND

We have reported the preventive effect of cimetidine, a histamine type 2 receptor antagonist, on decreased gastric mucosal blood flow induced by burn injury, a model of surgical stress.

OBJECTIVE

The present study was performed to assess the effects of cimetidine on gastric microcirculatory disturbance induced by surgical stress.

METHODS

Twelve male Wistar rats were anesthetized and a 30% full-thickness dorsal scald burn was inflicted. The total lengths of gastric erosions were measured using stereoscopic microscopy. Microvascular images in the basal region of the gastric mucosa were observed using an intravital microscope, and the diameters of venules and collecting venules were measured. Rolling leukocytes inside and along the venules, an indication of endothelial damage to the microcirculation, were observed. The rats were assigned to the cimetidine group or the control group (both, n = 6). Cimetidine 100 mg/kg was administered 30 minutes before and 2.5 hours after infliction of the burn injury. Animals in the control group were given only isotonic saline. Values are expressed as mean (SD).

RESULTS

Contraction of venules was easily observed in all rats in the control group. However, venular contraction was rarely observed in the cimetidine group. The total length of gastric erosions was significantly decreased in the cimetidine group compared with the control group (0.93 [0.58] μm vs 5.98 [5.18] μm, respectively; P < 0.05). The percentages of rolling leukocytes that passed the confluence of a prevenule and a venule were also significantly decreased in the cimetidine group compared with the control group (4.7% [7.3%] vs 22.6% [5.7%]; P < 0.01). The diameters of the venules and collecting venules were significantly larger in the cimetidine group (57.3 [6.8] μm and 75.9 [3.6] μm, respectively) than in the control group (30.9 [9.2] μm and 46.8 [8.0] μm, respectively) (both, P < 0.01).

CONCLUSIONS

The present study suggests that cimetidine may have a protective effect against gastric microcirculatory disturbances induced by burn injury, a model of surgical stress.

摘要

背景

我们已报道了组胺2型受体拮抗剂西咪替丁对烧伤诱导的胃黏膜血流量减少的预防作用，烧伤是一种手术应激模型。

目的

本研究旨在评估西咪替丁对手术应激诱导的胃微循环障碍的影响。

方法

将12只雄性Wistar大鼠麻醉后造成30%体表面积的背部全层烫伤。使用体视显微镜测量胃糜烂的总长度。用活体显微镜观察胃黏膜基底区域的微血管图像，并测量小静脉和集合小静脉的直径。观察小静脉内部及沿小静脉滚动的白细胞，这是微循环内皮损伤的一个指标。将大鼠分为西咪替丁组和对照组（每组n = 6）。在烧伤前30分钟和烧伤后2.5小时给予西咪替丁100 mg/kg。对照组动物仅给予等渗盐水。数值以平均值（标准差）表示。

结果

对照组所有大鼠均易于观察到小静脉收缩。然而，西咪替丁组很少观察到小静脉收缩。与对照组相比，西咪替丁组胃糜烂的总长度显著降低（分别为0.93 [0.58] μm和5.98 [5.18] μm；P < 0.05）。与对照组相比，西咪替丁组通过前小静脉和小静脉汇合处的滚动白细胞百分比也显著降低（4.7% [7.3%] 对22.6% [5.7%]；P < 0.01）。西咪替丁组小静脉和集合小静脉的直径显著大于对照组（分别为57.3 [6.8] μm和75.9 [3.6] μm）（分别为30.9 [9.2] μm和46.8 [8.0] μm）（均P < 0.01）。