一种蛋白酶抑制剂可减轻大鼠热损伤后早期的胃黏膜糜烂和微循环障碍。
A protease inhibitor attenuates gastric erosions and microcirculatory disturbance in the early period after thermal injury in rats.
作者信息
Yoshida M, Wakabayashi G, Ishikawa H, Otani Y, Shimazu M, Kubota T, Kumal K, Kurose I, Miura S, Ishii H, Kitajima M
机构信息
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
出版信息
J Gastroenterol Hepatol. 1998 Jan;13(1):104-8. doi: 10.1111/j.1440-1746.1998.tb00553.x.
The effects of camostat mesilate, a synthetic serine protease inhibitor on gastric microcirculation and active oxygen species generated by leucocytes from the gastric and jugular veins in the early period after thermal injury were assessed. Male Wistar rats were anaesthetized and a 30% full skin-thickness dorsal burn was inflicted. Camostat mesilate (100 mg/kg) was dissolved in distilled water and administered orally to rats 40 min before thermal injury (the camostat group). The control animals (the vehicle group) were administered distilled water orally. Rolling leucocytes as well as Monastral blue B deposits in venules were observed using in vivo microscopy. Active oxygen species were measured by chemiluminescence. Camostat mesilate decreased the total length of gastric erosion, venular deposits of Monastral blue B, and rolling of leucocytes in venules, and relatively increased luminol-dependent chemiluminescence activity generated by zymosan-stimulated leucocytes 15 min after thermal injury. These results suggest that serine proteases are involved in the formation of gastric erosions and gastric microcirculatory disturbance in the early period after thermal injury.
评估了合成丝氨酸蛋白酶抑制剂甲磺酸加贝酯对热损伤后早期胃微循环以及胃静脉和颈静脉白细胞产生的活性氧的影响。将雄性Wistar大鼠麻醉后，造成30%体表面积的背部全层烧伤。将甲磺酸加贝酯（100mg/kg）溶于蒸馏水中，在热损伤前40分钟经口给予大鼠（加贝酯组）。对照动物（赋形剂组）经口给予蒸馏水。使用体内显微镜观察滚动的白细胞以及小静脉中的丽春红B沉积。通过化学发光法测量活性氧。甲磺酸加贝酯减少了胃糜烂的总长度、丽春红B的小静脉沉积以及小静脉中白细胞的滚动，并相对增加了热损伤后15分钟酵母聚糖刺激的白细胞产生的鲁米诺依赖性化学发光活性。这些结果表明，丝氨酸蛋白酶参与了热损伤后早期胃糜烂的形成和胃微循环障碍。
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