Arora Shagun, Tandon Chanderdeep, Tandon Simran
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat Solan 173234, India.
ScientificWorldJournal. 2014 Feb 3;2014:452892. doi: 10.1155/2014/452892. eCollection 2014.
The purpose of this current study was to justify the incorporation of complementary and alternate medicine (CAM) in current cancer treatments. The major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells. Keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-Fluorouracil (5-FU), with certain CAM therapies previously reported to have anticancer activity. The parameters chosen for the study were based on antiproliferative and cytotoxic effects on normal, kidney epithelial cells (NRK-52E). The MTT assay, colony formation assay, DNA fragmentation, and differential staining using AO/EB, following treatment with either 5-FU or CAM therapies, were performed. The CAM therapies under study were various extracts of wheatgrass, roots of Achyranthes aspera (AA), mushroom extracts (Pleurotus ostreatus, Macrolepiota procera, and Auricularia polytricha), and a homeopathic drug, Ruta graveolens (Ruta). The results showed that treatment of normal cells with the CAM therapies led to minimum cell damage in comparison to 5-FU. This evidence-based study will lead to greater acceptance of alternative therapies against cancer.
本项研究的目的是证明在当前癌症治疗中纳入补充和替代医学（CAM）的合理性。抗癌药物的主要缺点是其非选择性杀伤，这最终会导致正常细胞的损耗。基于此作为我们研究的基础，我们努力将与已知化疗药物5-氟尿嘧啶（5-FU）相关的细胞毒性与先前报道具有抗癌活性的某些CAM疗法进行比较。本研究选择的参数基于对正常肾上皮细胞（NRK-52E）的抗增殖和细胞毒性作用。在用5-FU或CAM疗法处理后，进行MTT测定、集落形成测定、DNA片段化以及使用AO/EB的差异染色。所研究的CAM疗法包括小麦草的各种提取物、牛膝（AA）的根、蘑菇提取物（平菇、高大环柄菇和木耳）以及顺势疗法药物芸香（Ruta）。结果表明，与5-FU相比，用CAM疗法处理正常细胞导致的细胞损伤最小。这项基于证据的研究将使针对癌症的替代疗法得到更广泛的接受。
