Oswald R E, Papke R L, Lukas R J
Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Neurosci Lett. 1989 Jan 16;96(2):207-12. doi: 10.1016/0304-3940(89)90059-1.
Acetylcholine (ACh)-gated single channel events were studied on the TE671 human medulloblastoma clonal cell line by the use of the cell-attached patch clamp technique. Channel activity was detected (86% probability) in the presence of 0.1-2 microM ACh but not (0% probability) in the absence of agonist or in the presence of 1 microM alpha-bungarotoxin (Bgt). This effect of Bgt was reversible within 1 h. The most prominent channel type had a conductance of 50 pS. The kinetics of opening and closing of the channel were similar to that for skeletal muscle nicotinic acetylcholine receptors.
利用细胞贴附式膜片钳技术，在TE671人髓母细胞瘤克隆细胞系上研究了乙酰胆碱（ACh）门控单通道事件。在存在0.1 - 2微摩尔/升ACh的情况下检测到通道活性（概率为86%），而在无激动剂或存在1微摩尔/升α - 银环蛇毒素（Bgt）时未检测到通道活性（概率为0%）。Bgt的这种作用在1小时内是可逆的。最主要的通道类型电导为50皮西门子。该通道的开闭动力学与骨骼肌烟碱型乙酰胆碱受体的相似。
