乙型肝炎病毒感染进展过程中循环 CD4+和 CD8+T 细胞中程序性死亡受体-1 的表达及其与临床基线特征的相关性。

The expression of programmed death-1 in circulating CD4+ and CD8+ T cells during hepatitis B virus infection progression and its correlation with clinical baseline characteristics.

机构信息

The Affiliated Infectious Hospital of Soochow University, Suzhou, China. ; Key Laboratory of Infection and Immunity of Suzhou City, Suzhou, China.

Key Laboratory of Infection and Immunity of Suzhou City, Suzhou, China. ; Medical Biotechnology Institute, Medical College of Soochow University, Suzhou, China.

出版信息

Gut Liver. 2014 Mar;8(2):186-95. doi: 10.5009/gnl.2014.8.2.186. Epub 2013 Dec 24.

BACKGROUND/AIMS: Programmed death-1 (PD-1) expression was investigated in CD4(+) and CD8(+) T cells from hepatitis B virus (HBV)-infected patients at the chronic hepatitis B (CHB) infection, liver cirrhosis (LC), and hepatocellular carcinoma (HCC) stages.

METHODS

PD-1 expression in circulating CD4(+) and CD8(+) T cells was detected by flow cytometry. The correlations between PD-1 expression and HBV viral load, alanine aminotransaminase (ALT) levels and aspartate aminotransferase (AST) levels were analyzed using GraphPad Prism 5.0.

RESULTS

PD-1 expression in CD4(+) and CD8(+) T cells was significantly increased in both the CHB group and advanced-stage group (LC plus HCC). In the CHB group, PD-1 expression in both CD4(+) and CD8(+) T cells was positively correlated with the HBV viral load, ALT, and AST levels. However, in the LC plus HCC group, significant correlations between PD-1 expression and the clinical parameters were nearly absent.

CONCLUSIONS

PD-1 expression in peripheral CD4(+) and CD8(+) T cells is dynamic, changes with HBV infection progression, and is related to HBV viral load and liver function, especially in CHB. PD-1 expression could be utilized as a potential clinical indicator to determine the extent of virus replication and liver injury.

背景/目的：研究乙型肝炎病毒（HBV）感染者在慢性乙型肝炎（CHB）感染、肝硬化（LC）和肝细胞癌（HCC）阶段的 CD4(+)和 CD8(+)T 细胞中程序性死亡受体-1（PD-1）的表达情况。

方法

通过流式细胞术检测循环 CD4(+)和 CD8(+)T 细胞中的 PD-1 表达。使用 GraphPad Prism 5.0 分析 PD-1 表达与 HBV 病毒载量、丙氨酸转氨酶（ALT）水平和天冬氨酸转氨酶（AST）水平之间的相关性。

结果

CHB 组和晚期组（LC 加 HCC）的 CD4(+)和 CD8(+)T 细胞中的 PD-1 表达均显著增加。在 CHB 组中，CD4(+)和 CD8(+)T 细胞中的 PD-1 表达与 HBV 病毒载量、ALT 和 AST 水平呈正相关。然而，在 LC 加 HCC 组中，PD-1 表达与临床参数之间几乎不存在显著相关性。