The Affiliated Infectious Hospital of Soochow University, Suzhou, China. ; Key Laboratory of Infection and Immunity of Suzhou City, Suzhou, China.
Key Laboratory of Infection and Immunity of Suzhou City, Suzhou, China. ; Medical Biotechnology Institute, Medical College of Soochow University, Suzhou, China.
Gut Liver. 2014 Mar;8(2):186-95. doi: 10.5009/gnl.2014.8.2.186. Epub 2013 Dec 24.
BACKGROUND/AIMS: Programmed death-1 (PD-1) expression was investigated in CD4(+) and CD8(+) T cells from hepatitis B virus (HBV)-infected patients at the chronic hepatitis B (CHB) infection, liver cirrhosis (LC), and hepatocellular carcinoma (HCC) stages.
PD-1 expression in circulating CD4(+) and CD8(+) T cells was detected by flow cytometry. The correlations between PD-1 expression and HBV viral load, alanine aminotransaminase (ALT) levels and aspartate aminotransferase (AST) levels were analyzed using GraphPad Prism 5.0.
PD-1 expression in CD4(+) and CD8(+) T cells was significantly increased in both the CHB group and advanced-stage group (LC plus HCC). In the CHB group, PD-1 expression in both CD4(+) and CD8(+) T cells was positively correlated with the HBV viral load, ALT, and AST levels. However, in the LC plus HCC group, significant correlations between PD-1 expression and the clinical parameters were nearly absent.
PD-1 expression in peripheral CD4(+) and CD8(+) T cells is dynamic, changes with HBV infection progression, and is related to HBV viral load and liver function, especially in CHB. PD-1 expression could be utilized as a potential clinical indicator to determine the extent of virus replication and liver injury.
背景/目的：研究乙型肝炎病毒（HBV）感染者在慢性乙型肝炎（CHB）感染、肝硬化（LC）和肝细胞癌（HCC）阶段的 CD4(+)和 CD8(+)T 细胞中程序性死亡受体-1（PD-1）的表达情况。
通过流式细胞术检测循环 CD4(+)和 CD8(+)T 细胞中的 PD-1 表达。使用 GraphPad Prism 5.0 分析 PD-1 表达与 HBV 病毒载量、丙氨酸转氨酶（ALT）水平和天冬氨酸转氨酶（AST）水平之间的相关性。
CHB 组和晚期组（LC 加 HCC）的 CD4(+)和 CD8(+)T 细胞中的 PD-1 表达均显著增加。在 CHB 组中，CD4(+)和 CD8(+)T 细胞中的 PD-1 表达与 HBV 病毒载量、ALT 和 AST 水平呈正相关。然而，在 LC 加 HCC 组中，PD-1 表达与临床参数之间几乎不存在显著相关性。
外周血 CD4(+)和 CD8(+)T 细胞中 PD-1 的表达是动态变化的，随着 HBV 感染的进展而变化，与 HBV 病毒载量和肝功能有关，尤其是在 CHB 中。PD-1 表达可作为潜在的临床指标，用于判断病毒复制和肝损伤的程度。
