Wang Zhu, Liu Jun-Tian, Sun Wan-Sen, Li Rui-Ping, Wang Ye
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Feb;34(2):203-8.
To investigate the effect of Qufeng Tongluo Recipe (QTR) on the expression of desmin and CD2-associated protein (CD2AP) in adriamycin-induced nephropathy rats.
The adriamycin-induced nephropathy rat model was induced by a disposable intravenous injection of adriamycin. The model was successfully established after 3 weeks. Rats were then randomly divided into the blank control group (Group A, n =12), the model control group (Group B, n = 8), the small, medium, large dose QTR group (Group C, n = 8; Group D, n = 8; Group E, n = 8), and the positive control group (Group F, n = 8). From the fourth week normal saline was given to rats in Group A and Group B, QTR 1.0 g/mL, 2.1 g/mL, and 4.2 g/mL was respectively administered to those in Group C, D, and E. Prednisone 25 mg/kg was given to rats in Group F. All medication was performed by gastrogavage at 10 mL/kg, once daily, for 28 successive days. 24-h urinary protein excretion and sera biochemical indices were determined during medication. At the end of the experiment, ultrastructure was observed, mRNA expression of desmin, mRNA and protein of CD2AP were detected by Real-time PCR and Western blot.
(1) Compared with Group B, 24-h urinary protein excretion significantly decreased in Group C, D, E, and F (P < 0.05). (2) Compared with Group B, Alb in Group C, D, and E increased (P < 0.05) and TC significantly decreased (P < 0.05). TG significantly increased in Group F (P < 0.05). (3) Results of electron microscope showed, compared with Group B, the morphology of foot cells was improved to various degrees in Groups D, E, and F, especially the foot process structure and the number of foot processes were significantly improved, which was more obviously shown in Group D and Group E. (4) mRNA expression of desmin, mRNA and protein of CD2AP increased in adriamycin-induced nephropathy rats (P < 0.05). After intervention, when compared with Group B, mRNA expression of desmin and CD2AP were significantly lower in Group C, D, E, and F (P < 0.05). (5) Compared with Group A, expression of desmin and CD2AP significantly increased (P < 0.05). Compared with Group B, the expression of desmin protein were obviously lower in Group C, D, E, and F, and the protein expression of desmin obviously decreased in Group D, E, and F (P < 0.05). The protein expression of desmin and CD2AP gradually decreased in Group C, D, and E (P < 0.05). Compared with Group F, the expression of CD2AP protein obviously increased in Group C and D (P < 0.05); the expression of CD2AP protein obviously decreased in Group E (P < 0.05); the expression of desmin protein was higher in Group C, D, and E (P < 0.05).
QTR's therapeutic effect on adriamycin-induced nephropathy rats might be achieved through altered expression of desmin and CD2AP.
探讨祛风通络方（QTR）对阿霉素诱导的肾病大鼠结蛋白（desmin）和CD2相关蛋白（CD2AP）表达的影响。
一次性静脉注射阿霉素诱导建立阿霉素诱导的肾病大鼠模型。3周后模型成功建立。然后将大鼠随机分为空白对照组（A组，n = 12）、模型对照组（B组，n = 8）、QTR小剂量组、中剂量组、大剂量组（C组，n = 8；D组，n = 8；E组，n = 8）和阳性对照组（F组，n = 8）。从第4周起，A组和B组大鼠给予生理盐水，C组、D组和E组大鼠分别给予1.0 g/mL、2.1 g/mL和4.2 g/mL的QTR。F组大鼠给予25 mg/kg泼尼松。所有药物均按10 mL/kg经胃管给药，每日1次，连续给药28天。给药期间测定24小时尿蛋白排泄量和血清生化指标。实验结束时，观察超微结构，采用实时荧光定量PCR和蛋白质免疫印迹法检测desmin的mRNA表达、CD2AP的mRNA和蛋白表达。
（1）与B组比较，C组、D组、E组和F组24小时尿蛋白排泄量显著降低（P < 0.05）。（2）与B组比较，C组、D组和E组白蛋白（Alb）升高（P < 0.05），总胆固醇（TC）显著降低（P < 0.05）。F组甘油三酯（TG）显著升高（P < 0.05）。（3）电镜结果显示，与B组比较，D组、E组和F组足细胞形态有不同程度改善，尤其是足突结构和足突数量明显改善，D组和E组表现更明显。（4）阿霉素诱导的肾病大鼠desmin的mRNA表达、CD2AP的mRNA和蛋白表达增加（P < 0.05）。干预后，与B组比较，C组、D组、E组和F组desmin和CD2AP的mRNA表达显著降低（P < 0.05）。（5）与A组比较，desmin和CD2AP表达显著增加（P < 0.05）。与B组比较，C组、D组、E组和F组desmin蛋白表达明显降低，D组、E组和F组desmin蛋白表达明显下降（P < 0.05）。C组、D组和E组desmin和CD2AP蛋白表达逐渐降低（P < 0.05）。与F组比较，C组和D组CD2AP蛋白表达明显升高（P < 0.05）；E组CD2AP蛋白表达明显降低（P < 0.05）；C组、D组和E组desmin蛋白表达较高（P < 0.05）。
祛风通络方对阿霉素诱导的肾病大鼠的治疗作用可能是通过改变desmin和CD2AP的表达实现的。
