Castro Laura, Zhang Ruiyong, Muñoz Jesús A, González Felisa, Blázquez M Luisa, Sand Wolfgang, Ballester Antonio
a Department of Material Science and Metallurgical Engineering , Complutense University of Madrid , Madrid , Spain.
Biofouling. 2014;30(4):501-11. doi: 10.1080/08927014.2014.892586. Epub 2014 Mar 27.
The aim of this work was to investigate the production of extracellular polymeric substances (EPS) by Aeromonas hydrophila grown under anaerobic conditions. EPS composition was studied for planktonic cells, cells attached to carbon fibre supports using a soluble ferric iron source and cells grown with a solid ferric iron mineral (gossan). Conventional spectrophotometric methods, Fourier transform infrared (FTIR) and confocal laser scanning microscopy (CLSM) were used to determine the main components in the biofilm extracted from the cultures. The key EPS components were proteins, indicating their importance for electron transfer reactions. Carbohydrates were observed mostly on the mineral and contained terminal mannosyl and/or terminal glucose, fucose and N-acetylgalactosamine residues.
这项工作的目的是研究嗜水气单胞菌在厌氧条件下产生胞外聚合物（EPS）的情况。对浮游细胞、使用可溶性铁源附着在碳纤维载体上的细胞以及用固态铁矿物（铁帽）培养的细胞的EPS组成进行了研究。采用传统分光光度法、傅里叶变换红外光谱（FTIR）和共聚焦激光扫描显微镜（CLSM）来确定从培养物中提取的生物膜中的主要成分。关键的EPS成分是蛋白质，表明它们对电子转移反应的重要性。碳水化合物主要存在于矿物上，含有末端甘露糖基和/或末端葡萄糖、岩藻糖和N-乙酰半乳糖胺残基。
