Liu Ke, Ji Fei, Xu YiCe, Wang XiaoYu, Hou ZhaoHui, Yang ShiMing
Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital , Beijing.
Acta Otolaryngol. 2014 Jun;134(6):564-70. doi: 10.3109/00016489.2014.892631. Epub 2014 Mar 27.
Ototoxic gentamicin exposure does not disrupt the expression of myosin VIIa in the inner hair cells (IHCs) of mice, whereas gentamicin ototoxicity causes altered expression of otoferlin in IHCs, as well as parallel hearing threshold shifts.
To explore whether myosin VIIa and otoferlin in IHCs have different responses to gentamicin ototoxicity.
Lower dose treatment (100 mg/kg): adult C57 mice were continuously injected intraperitoneally with gentamicin once a day for 14 consecutive days. Dose-dependent gentamicin treatment: mice were injected intraperitoneally with differing doses (100, 200, and 300 mg/kg) once a day for 2 consecutive days. The hearing thresholds were detected by auditory brainstem response (ABR). Immunostaining and Western blotting were utilized to measure the manner of expression of myosin VIIa and otoferlin in IHCs.
Lower dose treatment: There were no significant differences among the control (day 0), and 4, 7, and 14 days after the ototoxicity exposure (p > 0.05). Dose-dependent gentamicin treatment: There were no significant differences among the control, 100, 200, and 300 mg/kg groups after the ototoxicity exposure (p > 0.05). In contrast, we found an altered expression of otoferlin in IHCs among the control (day 0), and 4, 7, and 14 days of exposure, when the mice were exposed to gentamicin ototoxicity (p > 0.05).
耳毒性庆大霉素暴露不会破坏小鼠内毛细胞（IHC）中肌球蛋白VIIa的表达，而庆大霉素耳毒性会导致IHC中 otoferlin 的表达改变，以及听力阈值平行变化。
探讨 IHC 中的肌球蛋白 VIIa 和 otoferlin 对庆大霉素耳毒性是否有不同反应。
低剂量治疗（100 mg/kg）：成年 C57 小鼠连续 14 天每天腹腔注射一次庆大霉素。剂量依赖性庆大霉素治疗：小鼠连续 2 天每天腹腔注射不同剂量（100、200 和 300 mg/kg）。通过听性脑干反应（ABR）检测听力阈值。利用免疫染色和蛋白质印迹法测量 IHC 中肌球蛋白 VIIa 和 otoferlin 的表达方式。
低剂量治疗：对照组（第 0 天）与耳毒性暴露后第 4、7 和 14 天之间无显著差异（p > 0.05）。剂量依赖性庆大霉素治疗：耳毒性暴露后，对照组、100、200 和 300 mg/kg 组之间无显著差异（p > 0.05）。相比之下，当小鼠暴露于庆大霉素耳毒性时，我们发现在对照组（第 0 天）以及暴露的第 4、7 和 14 天，IHC 中 otoferlin 的表达发生了改变（p > 0.05）。
