[伤寒及甲乙型副伤寒患者中各种检测感染性O抗原血症方法的诊断重要性]

[Diagnostic importance of various methods of determining infectious O-antigenemia in patients with typhoid and paratyphoid fevers A and B].

作者信息

Cherkasov V L, Rubtsov I V, Erovichenkov A A, Kulakov V N, Tregub A V

出版信息

Ter Arkh. 1988;60(11):35-8.

Abstract

Altogether 181 patients with typhoid and paratyphoid fever (54 with typhoid fever, 50 with paratyphoid fever type A, and 77 with paratyphoid fever type B) were investigated. Of them 108 (59.7%) patients were examined during the 1st week of disease. Serum specific O-antigens of typhoid and paratyphoid fever agents were determined by enzyme immunoassay (EIA), radioimmunoassay (RIA), and O-aggregate hemagglutination reaction (O-AHA), serum specific O-antibodies were determined by RDHA, EIA, RIA or O-AHA used during the 1st week of disease were twice as effective as RDHA. Combined use of EIA and O-AHA for the detection of serum specific O-antigens made it possible to diagnose typhoid fever in 90.91%, paratyphoid fever type A in 96.15%, and paratyphoid fever type B in 95.55% of cases.

摘要

共对181例伤寒和副伤寒患者（54例伤寒、50例甲型副伤寒和77例乙型副伤寒）进行了调查。其中108例（59.7%）患者在发病第1周接受了检查。采用酶免疫测定法（EIA）、放射免疫测定法（RIA）和O凝集素血凝反应（O - AHA）检测伤寒和副伤寒病原体的血清特异性O抗原，采用反向被动血凝试验（RDHA）、EIA、RIA或O - AHA检测血清特异性O抗体，发病第1周采用的方法比RDHA的效果好两倍。联合使用EIA和O - AHA检测血清特异性O抗原，可使90.91%的伤寒、96.15%的甲型副伤寒和95.55%的乙型副伤寒患者得到诊断。