三氯乙酸沉淀法
TCA precipitation.
作者信息
Koontz Laura
机构信息
Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD, USA.
出版信息
Methods Enzymol. 2014;541:3-10. doi: 10.1016/B978-0-12-420119-4.00001-X.
Abstract
Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels. TCA precipitation denatures the protein, so it should not be used if the protein must remain in its folded state (e.g., if you want to measure a biochemical activity of the protein).
摘要
蛋白质的三氯乙酸（TCA）沉淀法通常用于浓缩蛋白质样品或在进行SDS-PAGE或二维凝胶等下游应用之前去除包括盐和去污剂在内的污染物。TCA沉淀会使蛋白质变性，因此如果蛋白质必须保持折叠状态（例如，如果你想测量蛋白质的生化活性），则不应使用该方法。
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