蛋白质的凝胶过滤色谱法（尺寸排阻色谱法）。

Gel filtration chromatography (size exclusion chromatography) of proteins.

作者信息

Duong-Ly Krisna C, Gabelli Sandra B

机构信息

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

出版信息

Methods Enzymol. 2014;541:105-14. doi: 10.1016/B978-0-12-420119-4.00009-4.

DOI:10.1016/B978-0-12-420119-4.00009-4

PMID:24674066

Abstract

The protocol described here allows the student to construct a standard curve for a gel filtration column with a separation range of 5-250 kD. The size (hydrodynamic radius) of a protein species stable in a buffer containing Tris-HCl, NaCl, and DTT is determined using this column. Modifications may be made to the buffer to accommodate the protein of interest and the separation range of the column.

摘要

此处所描述的实验方案能让学生构建一个用于分离范围为5 - 250 kD的凝胶过滤柱的标准曲线。利用此柱可测定在含有Tris - HCl、NaCl和二硫苏糖醇（DTT）的缓冲液中稳定存在的蛋白质种类的大小（流体力学半径）。可对缓冲液进行修改，以适应目标蛋白质和柱子的分离范围。